Description

4-Nitrophenyl Phosphate Bis(Cyclohexylammonium) Salt is a specialized biochemical reagent primarily used as a chromogenic substrate for alkaline phosphatase (ALP) enzymes. This compound matters for its critical role in colorimetric assays, enabling the sensitive detection and quantification of ALP activity in research and diagnostic applications. It is needed by professionals in life science research, clinical diagnostics, and pharmaceutical development for applications such as ELISA, Western blotting, and enzyme kinetics studies.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): Serves as a key substrate for detecting alkaline phosphatase-conjugated antibodies, generating a measurable yellow color.
• Clinical Diagnostics: Used in automated and manual clinical chemistry analyzers for the determination of ALP levels in serum, a vital marker for liver and bone disorders.
• Molecular Biology Research: Essential for non-radioactive detection in techniques like Western blotting and nucleic acid hybridization (e.g., Southern/Northern blotting).
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify modulators of alkaline phosphatase activity for drug discovery.
• Biochemical Research: Used in enzyme kinetics studies to characterize the properties and inhibition of alkaline phosphatase.
• In-situ Hybridization (ISH): Acts as a substrate for colorimetric detection of specific DNA or RNA sequences in tissue samples.

Basic Information

Item	Detail
Product Name	4-Nitrophenyl Phosphate Bis(Cyclohexylammonium) Salt
CAS No.	14545-82-5
Molecular Formula	C24H44N3O6P
Molecular Weight	501.60 g/mol
Synonyms	p-Nitrophenyl Phosphate Disodium Salt (alternative salt form); PNPP; 4-NPP; Phosphoric Acid 4-Nitrophenyl Ester Bis(Cyclohexylammonium) Salt; Alkaline Phosphatase Substrate; pNPP; 4-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate (common hydrated form); PNPP, Disodium Salt
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Phosphate Bis(Cyclohexylammonium) Salt is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive diagnostic and research applications. Each lot is tested to confirm identity, purity, and performance as an enzyme substrate. A comprehensive Certificate of Analysis (COA) detailing specifications such as assay, absorbance characteristics, and functional performance in ALP assays is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Absorbance (1% Solution)	Meets performance criteria
Water Content (KF)	≤ 2.0%
Solubility (in Water)	Clear, colorless solution
Functional Test (ALP Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.