Description

Cytidine, [5-3H] is a tritium-labeled nucleoside analog essential for advanced biochemical and pharmacological research. Its core value lies in providing a highly specific radioactive tracer for studying nucleoside metabolism, nucleic acid synthesis, and cellular uptake mechanisms. This labeled compound is critically needed by researchers in pharmaceutical development, molecular biology, and oncology for conducting precise radioligand binding assays, autoradiography, and metabolic pathway tracing.

Application

• Metabolic Pathway Tracing: Used as a radioactive tracer to study the synthesis, salvage, and catabolism of pyrimidine nucleotides in vitro and in vivo.
• Nucleoside Transport Studies: Employed in competitive binding and uptake assays to characterize equilibrative and concentrative nucleoside transporters in cell membranes.
• Drug Mechanism of Action (MOA) Research: Facilitates the investigation of anticancer and antiviral nucleoside analogs by tracking their incorporation into DNA/RNA or competitive inhibition.
• Autoradiography: Serves as a probe for visualizing the localization and distribution of nucleoside uptake sites in tissue sections.
• Enzyme Kinetics Assays: Acts as a substrate for enzymes like cytidine deaminase and nucleoside kinases to determine kinetic parameters and inhibitor efficacy.
• Biochemical Screening: Utilized in high-throughput screening platforms to identify novel modulators of nucleoside-metabolizing enzymes.

Basic Information

Product Name	Cytidine, [5-3H]
CAS No.	14419-78-4
Molecular Formula	C9H13N3O5 (³H)
Molecular Weight	Contact for details
Synonyms	5-Tritio-cytidine; [5-³H]Cytidine; Cytidine tritiated at C-5; 1-β-D-Ribofuranosylcytosine, [5-3H]-; 4-Amino-1-β-D-ribofuranosyl-2(1H)-pyrimidinone, [5-3H]-; Tritiated Cytidine; ³H-Cytidine; Cytidine-5-³H
EINECS	Contact for details

Quality Control

Every batch of Cytidine, [5-3H] is manufactured and tested to meet exacting standards for radiochemical purity and specific activity. Quality assurance includes rigorous analysis via HPLC with radiometric detection to confirm identity, purity, and isotopic labeling position. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity (Ci/mmol or GBq/mmol), radiochemical purity, chemical purity, and solvent specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at ≤ -20°C or as specified on the label and COA. The product is supplied in a suitable organic solvent (e.g., ethanol/water mixture); ensure proper laboratory safety protocols for handling radioactive and organic materials are followed. Store in a designated area for radioactive materials in accordance with local regulations.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	10-30 Ci/mmol (370-1110 GBq/mmol) (Typical)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol: Water (7:3 v/v) or as specified
Concentration	1 mCi/mL (37 MBq/mL) (Typical)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.