Description

Indoxyl Phosphate is a key biochemical reagent widely used as a chromogenic substrate for enzyme detection. This compound is critical for research and diagnostic applications where precise and sensitive detection of enzymatic activity is required. It is primarily utilized by professionals in the life sciences, clinical diagnostics, and pharmaceutical development sectors for assays involving alkaline phosphatase and other phosphatases.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): Serves as a sensitive chromogenic substrate for detecting alkaline phosphatase conjugates.
• Clinical Diagnostics: Used in automated analyzers and manual test kits for the determination of alkaline phosphatase levels in serum, a key diagnostic marker.
• Molecular Biology Research: Employed in blotting techniques (e.g., Western, Southern) for the colorimetric detection of target proteins or nucleic acids.
• Histochemistry & Cytochemistry: Acts as a substrate for locating and visualizing alkaline phosphatase activity in tissue sections and cell cultures.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) assays to identify potential enzyme inhibitors or activators.
• Water & Environmental Testing: Applied in certain enzymatic methods for detecting bacterial contamination or specific analytes.
• Biochemical Reagent Manufacturing: A core component in the formulation of diagnostic kits and research-grade biochemicals.

Basic Information

Product Name	Indoxyl Phosphate
CAS No.	13822-19-0
Molecular Formula	C8H8NO4P
Molecular Weight	213.13 g/mol
Synonyms	3-Indolyl phosphate; Indoxyl phosphate disodium salt (common salt form); 5-Indolyl phosphate; Phosphoric acid indol-3-yl ester; 3-Indoxyl phosphate; ALP Substrate; Alkaline Phosphatase Substrate; Indol-3-yl dihydrogen phosphate
EINECS	Contact for details

Quality Control

Our Indoxyl Phosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR), to ensure it meets the high standards required for sensitive diagnostic and research applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation due to moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, clear solution
Heavy Metals	< 10 ppm
Residue on Ignition	< 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.