Description

Transglutaminase 5 is a calcium-dependent enzyme belonging to the transglutaminase family, which catalyzes the formation of covalent bonds between proteins. This specific isoform plays a critical role in the formation of the cornified cell envelope in the epidermis, contributing to skin barrier function and integrity. It is an essential biochemical reagent for research in dermatology, cell biology, and protein cross-linking studies. Industrial and academic researchers utilize this high-purity enzyme to investigate keratinocyte differentiation, skin diseases, and novel biomaterial applications.

Application

• Biomedical Research: Fundamental studies on epidermal differentiation, skin barrier formation, and the pathophysiology of skin disorders such as ichthyosis.
• Enzymology Studies: Investigation of transglutaminase enzyme kinetics, substrate specificity, and calcium-dependent activation mechanisms.
• Protein Engineering: Used as a tool for site-specific protein cross-linking, labeling, and the development of protein-based biomaterials and hydrogels.
• Diagnostic Development: Potential use in the development of assays and kits for detecting autoimmune responses related to transglutaminase enzymes.
• Cell Culture & Tissue Models: Application in advanced 3D skin tissue modeling and studying cornification processes in vitro.
• Pharmaceutical Screening: Target enzyme for screening potential therapeutic modulators in dermatological and autoimmune drug discovery pipelines.

Basic Information

Product Name	Transglutaminase 5
CAS No.	13497-24-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Protein-glutamine γ-glutamyltransferase 5; TGase 5; TGM5; Transglutaminase-5; Keratinocyte transglutaminase; Epidermal transglutaminase; TGK; EC 2.3.2.13
EINECS	Contact for details

Quality Control

Our Transglutaminase 5 is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot is characterized for purity, specific activity, and absence of contaminating proteases. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications such as activity units/mg, protein content, and buffer composition. We adhere to relevant guidelines for research-grade biochemicals to support reproducible scientific results.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. Aliquot the product upon receipt for single-use applications to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> X U/mg protein (Contact for details)
Purity (SDS-PAGE)	> 90%
Protein Content (Bradford)	Reported on COA
Endotoxin Level (LAL)	< 1.0 EU/μg
Buffer Composition	Tris, NaCl, Glycerol, etc. (Specified on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.