Description

Phenolphthalein Monophosphate is a key chromogenic substrate used in enzymatic assays for the detection of alkaline phosphatase activity. Its high sensitivity and specificity make it an indispensable reagent for precise biochemical analysis and diagnostic applications. This compound is primarily utilized by manufacturers and research laboratories in the in-vitro diagnostics (IVD), life science research, and pharmaceutical quality control sectors.

Application

• Primary Substrate in Clinical Diagnostics: Used in automated analyzers and ELISA kits for the quantitative measurement of alkaline phosphatase (ALP) in serum, a critical marker for liver and bone disorders.
• Immunoassay Development: Serves as a chromogenic agent in enzyme-linked immunosorbent assays (ELISA) and other immunochemical staining procedures.
• Research & Development: Employed in biochemical research to study enzyme kinetics, inhibitor screening, and cellular signaling pathways involving phosphatases.
• Histology & Cytology: Acts as a substrate in histochemical staining for the localization of alkaline phosphatase activity in tissue sections and cell cultures.
• Water & Environmental Testing: Applied in certain enzymatic methods for the detection of bacterial contamination or specific enzymatic activities in environmental samples.
• Pharmaceutical QC: Used in quality control testing of biopharmaceuticals where alkaline phosphatase is a relevant process or contaminant enzyme.

Basic Information

Product Name	Phenolphthalein Monophosphate
CAS No.	13306-67-7
Molecular Formula	C20H15O8P
Molecular Weight	414.30 g/mol
Synonyms	Phenolphthalein Phosphate; Phenolphthalein Diphosphate (Disodium Salt); 3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone Monophosphate; PPP; ALP Substrate; Alkaline Phosphatase Substrate; Diagnostic Reagent; Chromogenic Phosphatase Substrate
EINECS	Contact for details

Quality Control

Our Phenolphthalein Monophosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and batch-to-batch reproducibility critical for diagnostic and research applications. We provide comprehensive Certificates of Analysis (COA) detailing identity, purity, and functional performance specifications. Our quality standards are designed to meet the demands of GMP/ISO 13485 environments for in-vitro diagnostic manufacture.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation due to moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, buffer solutions
Functional Activity	Conforms to reference standard in ALP assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.