Description

2-Nitrophenyl-n-Acetyl-β-D-Glucosaminide is a specialized chromogenic substrate used in enzymatic assays. This compound matters for its high specificity in detecting and quantifying the activity of β-N-acetylglucosaminidase enzymes. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies developing enzyme-based detection systems.

Application

• Enzymatic Research: As a chromogenic substrate for studying the kinetics and inhibition of β-N-acetylglucosaminidase (NAGase) enzymes.
• Clinical Diagnostics: A key component in diagnostic assays for detecting lysosomal storage disorders, such as Tay-Sachs and Sandhoff diseases.
• Microbiology: Used in microbial identification systems to detect specific bacterial enzyme activities.
• Biochemical Reagent: Serves as a critical raw material in the formulation of research-grade and in-vitro diagnostic (IVD) reagent kits.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) campaigns to identify potential therapeutic modulators of glycosidase activity.
• Quality Control: Used as a reference standard in the quality control of enzyme preparations and diagnostic products.

Basic Information

Product Name	2-Nitrophenyl-n-Acetyl-β-D-Glucosaminide
CAS No.	13264-92-1
Molecular Formula	C14H18N2O8
Molecular Weight	342.30 g/mol
Synonyms	4-Nitrophenyl N-acetyl-β-D-glucosaminide; p-Nitrophenyl N-acetyl-β-D-glucosaminide; PNP-NAG; NAG; 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside; 2-Acetamido-2-deoxy-4-nitrophenyl-β-D-glucopyranoside; β-N-Acetylglucosaminide 4-nitrophenyl; p-Nitrophenyl β-N-acetyl-D-glucosaminide
EINECS	Contact for details

Quality Control

Our 2-Nitrophenyl-n-Acetyl-β-D-Glucosaminide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.