Description

4-Nitrophenyl2-Acetamido-3,4,6-Tri-o-Acetyl-2-Deoxy-A-D-Glucopyranoside is a key glycosylated intermediate and chromogenic substrate widely used in biochemical research and synthesis. This compound is valued for its role as a specific substrate for glycosidase enzymes, enabling the study of enzyme kinetics and activity. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent sectors for developing enzyme assays and synthesizing complex glycoconjugates.

Application

• Glycosidase Enzyme Assays: Acts as a chromogenic substrate for the detection and kinetic analysis of β-N-acetylglucosaminidase and related enzymes.
• Biochemical Research: Used as a critical tool in studying carbohydrate metabolism, enzyme inhibition, and glycoprotein processing pathways.
• Diagnostic Reagent Synthesis: Serves as a key building block in the production of reagents for clinical diagnostics and research kits.
• Pharmaceutical Intermediate: Employed in the synthesis of more complex glycosides, glycomimetics, and potential therapeutic agents.
• Glycobiology Studies: Facilitates research into cell-surface interactions, signaling, and the biological roles of carbohydrates.
• Quality Control in Enzyme Production: Used to standardize and assess the activity of commercially produced glycosidase enzymes.

Basic Information

Product Name	4-Nitrophenyl2-Acetamido-3,4,6-Tri-o-Acetyl-2-Deoxy-A-D-Glucopyranoside
CAS No.	13089-23-1
Molecular Formula	C₂₁H₂₆N₂O₁₂
Molecular Weight	522.44 g/mol
Synonyms	p-Nitrophenyl N-acetyl-β-D-glucosaminide, per-O-acetylated; 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside triacetate; PNP-β-GlcNAc triacetate; 4-Nitrophenyl N-acetyl-β-D-glucosaminide triacetate; 2-Acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-D-glucopyranosyl 4-nitrophenyl ether; NAG triacetate p-nitrophenyl glycoside; β-GlcNAc-ONp triacetate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl2-Acetamido-3,4,6-Tri-o-Acetyl-2-Deoxy-A-D-Glucopyranoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and IR spectroscopy, to ensure it meets the high standards required for research and synthesis. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.