Description

Digitonin is a high-purity, non-ionic saponin glycoside derived from the seeds of the foxglove plant (*Digitalis purpurea*). This compound is a critical reagent in biochemical research, valued for its unique ability to selectively permeabilize cell membranes without disrupting intracellular organelles. It is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries who require reliable, high-quality materials for cell fractionation, cholesterol determination, and protein extraction protocols.

Application

• Cell Membrane Permeabilization: A standard tool for selectively permeabilizing the plasma membrane while leaving organelle membranes intact, crucial for immunofluorescence, in situ hybridization, and introducing antibodies or other molecules into cells.
• Cholesterol Assay & Research: Used to solubilize membrane cholesterol, making it a key component in enzymatic and colorimetric assays for quantifying free cholesterol in serum, tissues, and cultured cells.
• Subcellular Fractionation: Employed in protocols to isolate specific organelles, such as mitochondria or nuclei, by creating pores in the plasma membrane to release cytosolic contents.
• Protein Extraction & Solubilization: Facilitates the extraction of membrane-associated proteins and receptors by disrupting lipid-lipid and lipid-protein interactions.
• Diagnostic Reagent Formulation: Incorporated into diagnostic kits and reagents for clinical chemistry applications, particularly those measuring lipid profiles.
• Pharmaceutical Research: Used in drug discovery and development studies investigating cholesterol metabolism, lipid rafts, and membrane protein function.

Basic Information

Item	Detail
Product Name	Digitonin
CAS No.	11024-24-1
Molecular Formula	C56H92O29
Molecular Weight	1229.31 g/mol
Synonyms	Digitalin; Digitonoside; (3β,5β,25R)-3-[(2,6-Dideoxy-4-O-β-D-glucopyranosyl-β-D-ribo-hexopyranosyl)oxy]-14-hydroxycard-20(22)-enolide 3-[(2,6-Dideoxy-β-D-ribo-hexopyranosyl-(1→4)-2,6-dideoxy-β-D-ribo-hexopyranosyl-(1→4)-2,6-dideoxy-β-D-ribo-hexopyranosyl)oxy]-; Digitalis saponin
EINECS	234-258-9

Quality Control

Our Digitonin is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and specific tests for solubility and performance in cholesterol assays. A Certificate of Analysis (COA) detailing all specifications and results is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect performance and stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0%
Solubility	Soluble in water, methanol, and DMSO
Loss on Drying	≤ 5.0 %
Residue on Ignition	≤ 0.5 %
Heavy Metals	≤ 20 ppm
Biological Activity (Cholesterol Assay)	Conforms to reference standard performance

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.