Description

4-Nitrophenyl-β-D-Glucuronide is a chromogenic substrate specifically designed for the detection and quantification of β-glucuronidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by biotechnology, pharmaceutical, and clinical diagnostic laboratories for enzyme-linked assays, high-throughput screening, and microbiological identification.

Application

• Enzymatic Substrate for β-Glucuronidase: Used as a key reagent in biochemical assays to measure β-glucuronidase activity, a common reporter system in molecular biology.
• Microbiological Testing: Employed in culture media for the detection of E. coli and other coliform bacteria that express the GUS gene, a standard method in water safety and food microbiology.
• High-Throughput Screening (HTS): Serves as a chromogenic probe in drug discovery platforms to identify inhibitors or activators of β-glucuronidase.
• Diagnostic Kits: An essential component in clinical diagnostic kits for measuring specific enzyme levels related to certain metabolic conditions or bacterial infections.
• Research Tool: Used in academic and industrial research to study gene expression (GUS gene fusion), lysosomal storage disorders, and gut microbiome metabolism.
• Quality Control in Biotechnology: Applied in the quality assurance of recombinant enzymes and in-process monitoring of fermentation where β-glucuronidase is involved.

Basic Information

Product Name	4-Nitrophenyl-β-D-Glucuronide
CAS No.	10344-94-2
Molecular Formula	C12H13NO9
Molecular Weight	315.23 g/mol
Synonyms	p-Nitrophenyl β-D-Glucopyranosiduronic Acid; p-Nitrophenyl β-D-Glucuronide; 4-NPG; PNPG; 4-Nitrophenyl β-D-Glucuronide; β-4-Nitrophenyl Glucuronide; Glucuronide, 4-nitrophenyl-; 4-Nitrophenylglucuronide
EINECS	233-745-5

Quality Control

Our 4-Nitrophenyl-β-D-Glucuronide is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and NMR, and specific tests for residual solvents and moisture. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with your research and production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	-50° to -55° (c=1 in H2O)
Solubility	Clear, colorless solution at 10 mg/mL in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.