Description

2'-Nitrophenyl-2-Acetamido-2-Deoxy-α-D-Glucopyranoside is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound matters for its high specificity and sensitivity in detecting and quantifying glycosidase enzyme activity, particularly N-acetyl-β-D-glucosaminidase. It is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors who require reliable tools for enzyme kinetics and inhibitor screening.

Application

• Enzymatic Assay Substrate: Primary substrate for the detection and kinetic analysis of N-acetyl-β-D-glucosaminidase (NAGase) and related glycosidases.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for clinical chemistry, including tests for kidney function and certain lysosomal storage disorders.
• Biochemical Research: A critical tool in academic and industrial research for studying enzyme mechanisms, inhibition, and carbohydrate-active enzymes (CAZymes).
• Pharmaceutical Development: Employed in high-throughput screening (HTS) campaigns to identify potential therapeutic inhibitors of target glycosidases.
• Quality Control (QC) Testing: Used in QC laboratories for the potency testing of enzyme preparations and related biopharmaceutical products.
• Microbiology: Can be applied in studies of bacterial and fungal enzymes involved in pathogenesis or biomass degradation.

Basic Information

Product Name	2'-Nitrophenyl-2-Acetamido-2-Deoxy-α-D-Glucopyranoside
CAS No.	10139-01-2
Molecular Formula	C14H18N2O8
Molecular Weight	342.30 g/mol
Synonyms	p-Nitrophenyl N-acetyl-α-D-glucosaminide; 4-Nitrophenyl 2-acetamido-2-deoxy-α-D-glucopyranoside; α-pNP-GlcNAc; NAG-α-pNP; 2-Acetamido-2-deoxy-α-D-glucopyranosyl 4-nitrobenzoate; 4-Nitrophenyl α-N-acetylglucosaminide; α-4-Nitrophenyl N-acetylglucosaminide; PNP-α-GlcNAc
EINECS	Contact for details

Quality Control

Our 2'-Nitrophenyl-2-Acetamido-2-Deoxy-α-D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.