Description

Naphthol As-Bi Phosphate CAS NO 1919-91-1 is a specialized biochemical reagent primarily used as a substrate for the detection of alkaline phosphatase activity in enzyme-linked immunoassays and histochemical staining. Its value lies in its ability to produce a highly colored, insoluble precipitate upon enzymatic hydrolysis, enabling precise localization and quantification in diagnostic and research applications. This compound is essential for manufacturers and research laboratories in the in-vitro diagnostics (IVD), life science research, and histopathology sectors.

Application

• Primary substrate for Alkaline Phosphatase (ALP) in ELISA (Enzyme-Linked Immunosorbent Assay) and other immunoassay kits.
• Histochemical staining agent for visualizing alkaline phosphatase activity in tissue sections, particularly in bone, liver, and placenta studies.
• Key component in chromogenic in-situ hybridization (CISH) and other molecular pathology techniques.
• Used in western blotting and membrane-based assays for protein detection.
• Essential for diagnostic kit manufacturing for clinical chemistry and immunology.
• Research tool in cell biology and enzymology to study phosphatase signaling pathways.

Basic Information

Product Name	Naphthol As-Bi Phosphate
CAS No.	1919-91-1
Molecular Formula	C23H18NO6P
Molecular Weight	435.36 g/mol
Synonyms	Naphthol AS-BI Phosphate; 3-Hydroxy-4-nitrophenyl 2-Naphthyl Phosphate; Naphthol AS-BI Phosphoric Acid; 2-Naphthyl 3-Hydroxy-4-nitrophenyl Phosphate; Phosphoric Acid 2-Naphthyl 3-Hydroxy-4-nitrophenyl Ester; AS-BI Phosphate; Naphthol ASBI Phosphate; Alkaline Phosphatase Substrate
EINECS	217-645-7

Quality Control

Our Naphthol As-Bi Phosphate is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical diagnostic and research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in enzymatic assays. We support compliance with ISO 13485 for in-vitro diagnostic manufacturing and relevant pharmacopeial guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%
Enzymatic Activity	Suitable for use as ALP substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.