Description

3Alpha,20Alpha-Dihydroxy-5Beta-Pregnane 3-Glucuronide is a high-purity conjugated steroid metabolite, specifically the 3-O-glucuronide derivative of a dihydroxylated pregnane. This compound is of significant value in biochemical research and pharmaceutical development as a critical reference standard for analytical method validation and metabolic pathway studies. It is primarily needed by research institutions, diagnostic manufacturers, and pharmaceutical companies engaged in steroid hormone analysis, biomarker discovery, and the development of assays for endocrine function.

Application

• Pharmaceutical Reference Standard: Serves as a certified primary standard for the quantitative analysis of steroid metabolites in drug development and bioequivalence studies.
• Clinical Diagnostics: Used in the calibration and quality control of LC-MS/MS and immunoassay systems for measuring steroid hormone profiles in patient samples.
• Metabolic Research: A crucial tool for investigating phase II metabolism (glucuronidation) of pregnane steroids and studying related enzyme kinetics.
• Biomarker Development: Employed in research to identify and validate specific steroid glucuronides as biomarkers for various physiological and pathological conditions.
• Forensic Toxicology: Acts as a reference material in the confirmation and quantification of steroid metabolites in anti-doping control and forensic analyses.
• Enzyme Substrate: Used in in vitro assays to study the activity and inhibition of UDP-glucuronosyltransferase (UGT) enzymes.

Basic Information

Item	Details
Product Name	3Alpha,20Alpha-Dihydroxy-5Beta-Pregnane 3-Glucuronide
CAS No.	1852-49-9
Molecular Formula	C27H42O9
Molecular Weight	510.62 g/mol
Synonyms	5β-Pregnane-3α,20α-diol 3-Glucuronide; 3α,20α-Dihydroxy-5β-pregnan-3-yl-β-D-glucopyranosiduronic Acid; Pregnane-3α,20α-diol 3-Glucuronide; 3α,20α-Dihydroxy-5β-pregnane 3-(β-D-Glucopyranosiduronic Acid); 5β-Pregnan-3α,20α-diol 3-Glucuronide; 3-Glucuronide of 5β-Pregnane-3α,20α-diol; PDG-3G; Contact for additional synonyms.
EINECS	Contact for details

Quality Control

Our 3Alpha,20Alpha-Dihydroxy-5Beta-Pregnane 3-Glucuronide is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and determination of residual solvents and moisture content. We provide a comprehensive Certificate of Analysis (COA) with each shipment, detailing all test results against established specifications to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and purity. For long-term storage, consider desiccant conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.