Description

Z-Gly-Gly-Arg-Betana Hcl is a synthetic peptide derivative containing a benzothiazole-based fluorogenic leaving group (Betana). This compound is primarily valued as a highly sensitive and specific substrate for the detection and kinetic analysis of serine protease activity, particularly thrombin. It is an essential tool for researchers and quality control laboratories in the life sciences and pharmaceutical development sectors requiring precise enzymatic assays.

Application

• Protease Activity Assays: A fluorogenic substrate for the continuous, real-time monitoring of thrombin and related serine protease activity in biochemical research.
• Drug Discovery & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel anticoagulant or procoagulant drug candidates.
• Clinical Diagnostics: Employed in the development of in vitro diagnostic tests for measuring blood coagulation parameters and detecting clotting disorders.
• Enzyme Kinetics Studies: Essential for determining kinetic parameters (Km, kcat) of proteases, providing insights into enzyme mechanism and specificity.
• Quality Control in Biologics: Used to assess the potency and consistency of therapeutic enzymes or products derived from biological processes.
• Biochemical Research Reagent: A critical component in studying protease signaling pathways, cell biology, and physiological processes involving proteolytic cleavage.

Basic Information

Product Name	Z-Gly-Gly-Arg-Betana Hcl
CAS No.	1442-79-1
Molecular Formula	C31H39N9O7S · xHCl
Molecular Weight	681.77 g/mol (free base)
Synonyms	Benzoyl-Gly-Gly-Arg-2-Aminobenzothiazole Hcl; Z-Gly-Gly-Arg-ABT Hcl; Boc-Gly-Gly-Arg-AMC (related fluorophore); Thrombin Substrate I, Fluorogenic; Benzoyl-Gly-Gly-Arg-AMC (alternative fluorophore); Nα-Benzoyl-Glycyl-glycyl-L-arginine 2-aminobenzothiazole hydrochloride; Protease Fluorogenic Substrate
EINECS	Contact for details

Quality Control

Our Z-Gly-Gly-Arg-Betana Hcl is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay systems. We adhere to rigorous quality testing protocols to meet the exacting standards required for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with structure
Purity (HPLC)	≥ 95.0% (Area Percent)
Assay (Enzymatic)	≥ 90% of theoretical activity vs. reference
Water Content (KF)	≤ 5.0 %
Solubility	Clear, colorless solution in DMSO or assay buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.