Description

Z-Leu-Pna is a specialized peptide derivative, specifically a protected leucine para-nitroanilide substrate. This compound is a critical reagent in biochemical and pharmaceutical research, valued for its role as a chromogenic substrate for various proteolytic enzymes. It is primarily utilized by research institutions and pharmaceutical companies engaged in enzyme kinetics studies, protease activity assays, and high-throughput screening. The product is supplied with a focus on high purity and batch-to-batch consistency to ensure reliable experimental results.

Application

• Protease & Enzyme Research: Acts as a chromogenic substrate for quantifying the activity of proteases like chymotrypsin, subtilisin, and other serine or cysteine proteases.
• Biochemical Assay Development: Used in the development and optimization of colorimetric and spectrophotometric assays to study enzyme kinetics and inhibition.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors as drug candidates.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for detecting specific enzymatic activities in clinical samples.
• Academic & Industrial R&D: Essential for fundamental research in biochemistry, molecular biology, and medicinal chemistry within university labs and corporate R&D centers.

Basic Information

Item	Details
Product Name	Z-Leu-Pna
CAS No.	1174-27-2
Molecular Formula	C20H23N3O5
Molecular Weight	385.42 g/mol
Synonyms	Z-Leu-pNA; Cbz-Leu-pNA; N-Carbobenzoxy-L-leucine p-nitroanilide; Benzyloxycarbonyl-L-leucine 4-nitroanilide; L-Leucine, N-[(phenylmethoxy)carbonyl]-, 4-nitroanilide; Chz-Leu-pNA; Substrate for chymotrypsin; Protease substrate 1174-27-2
EINECS	Contact for details

Quality Control

Every batch of Z-Leu-Pna is manufactured and tested under a strict quality management system. We ensure high purity and identity through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to guarantee compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Heavy Metals	< 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.