Description

o-Nitrophenyl-1-Thio-β-D-Galactopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of β-galactosidase activity, a common reporter enzyme in molecular biology and diagnostic applications. It is an essential reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic kit production industries.

Application

• β-Galactosidase Assays: Primary chromogenic substrate for the colorimetric detection of β-galactosidase (β-Gal) activity in enzymatic studies.
• Molecular Biology Research: Used as a reporter gene substrate in cloning, transfection, and gene expression experiments (e.g., lacZ gene system).
• Diagnostic Kit Manufacturing: A key component in the formulation of diagnostic kits for microbiology and genetic testing.
• Enzyme Characterization: Employed in biochemical research for kinetic studies and inhibitor screening of β-galactosidase enzymes.
• Cell-Based Screening: Utilized in high-throughput screening (HTS) assays within drug discovery pipelines.
• Food & Environmental Testing: Applied in methods for detecting bacterial contamination (e.g., E. coli) that express β-galactosidase.

Basic Information

Product Name	o-Nitrophenyl-1-Thio-β-D-Galactopyranoside
CAS No.	1158-17-4
Molecular Formula	C12H15NO7S
Molecular Weight	317.32 g/mol
Synonyms	ONPG-S; o-Nitrophenyl-β-D-thiogalactopyranoside; 2-Nitrophenyl 1-Thio-β-D-galactopyranoside; ONP-β-D-thiogalactoside; 1-Thio-β-D-galactopyranoside 2-nitrophenyl; Thiogalactoside ONP; β-Galactosidase substrate (chromogenic); 2-Nitrophenyl thiogalactoside
EINECS	Contact for details

Quality Control

Our o-Nitrophenyl-1-Thio-β-D-Galactopyranoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is analyzed to confirm identity, purity, and performance in enzymatic assays. Certificates of Analysis (COA) detailing specifications such as HPLC purity, melting point, and extinction coefficient are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to minimize exposure to moisture and light. For long-term storage, consider desiccants and inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	185 - 190 °C (dec.)
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Extinction Coefficient	~3,500 M-1cm-1 at 405 nm (in assay buffer)
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.