Description

4-Nitrophenyl-β-D-Fucopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of specific glycosidase enzymes, such as β-fucosidase, due to its ability to release a colored 4-nitrophenolate ion upon hydrolysis. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic reagent manufacturing sectors for biochemical research and enzyme activity profiling.

Application

• Enzymatic Substrate for β-Fucosidase: Primary chromogenic substrate for the spectrophotometric assay of β-fucosidase activity in research and diagnostic applications.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and characterization of glycosidase enzyme families.
• Diagnostic Reagent Manufacturing: A key raw material in the formulation of diagnostic kits for the detection of specific enzyme deficiencies or microbial enzymes.
• Microbiology: Employed in microbial identification systems that rely on specific glycosidase profiles for bacterial differentiation.
• Pharmaceutical QC: Used in quality control testing of enzyme-based pharmaceuticals or in-process monitoring of fermentation broths.
• Glycobiology Studies: A tool for investigating fucose metabolism and the role of fucosidases in various biological pathways.

Basic Information

Product Name	4-Nitrophenyl-β-D-Fucopyranoside
CAS No.	1154-94-5
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	p-Nitrophenyl β-D-fucopyranoside; 4-Nitrophenyl β-D-fucoside; β-D-Fucopyranoside, 4-nitrophenyl; PNPGal (related analog); pNP-β-D-Fuc; 4-Nitrophenyl-β-D-fucopyranoside; β-4-Nitrophenyl fucopyranoside; Fucosidase substrate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-β-D-Fucopyranoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be kept in a desiccated environment to maintain stability and prevent hydrolysis.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.