Description

Thymine, [Methyl-3H] is a tritium-labeled derivative of the nucleobase thymine, a fundamental building block of DNA. This radiolabeled compound is essential for conducting precise tracer studies in biochemical and pharmacological research, enabling the detailed investigation of DNA synthesis, metabolism, and repair mechanisms. It is a critical reagent for pharmaceutical R&D, academic institutions, and diagnostic developers focused on oncology, virology, and molecular biology.

Application

• DNA Synthesis & Metabolism Research: Used as a tracer to study the kinetics and pathways of DNA replication and turnover in cell cultures and animal models.
• Oncology & Anticancer Drug Development: Employed in assays to evaluate the efficacy and mechanism of action of chemotherapeutic agents that target DNA synthesis.
• Virology Studies: Applied in research on viral replication cycles, particularly for DNA viruses, to understand infection mechanisms and screen antiviral compounds.
• Autoradiography & Imaging: Serves as a probe for autoradiographic techniques to localize DNA synthesis activity in tissues and cellular compartments.
• Biochemical Pathway Analysis: Utilized in metabolic flux studies to trace the incorporation of thymine into nucleic acids and related metabolites.
• Quality Control for Radiopharmaceuticals: Acts as a reference standard in the development and testing of novel radiolabeled diagnostic or therapeutic agents.

Basic Information

Product Name	Thymine, [Methyl-3H]
CAS No.	1148-63-6
Molecular Formula	C5H43H2N2O2
Molecular Weight	128.1 g/mol (nominal, for unlabeled thymine)
Synonyms	5-Methyl-2,4(1H,3H)-pyrimidinedione, [Methyl-3H]; [3H]Thymine; 2,4-Dihydroxy-5-methylpyrimidine, tritiated; 5-Methyluracil, [Methyl-3H]; Thymine, methyl-tritiated; Radiolabeled Thymine; DNA Base, Tritiated
EINECS	Contact for details

Quality Control

Every batch of Thymine, [Methyl-3H] is manufactured and analyzed under strict quality systems. Product specifications, including radiochemical purity (typically >97% by HPLC), specific activity, and chemical purity, are rigorously verified. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with each shipment to ensure traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as specified on the label or COA. This product is strictly light-sensitive (protect from all light exposure). Handle and store in accordance with all applicable radiation safety regulations. Keep away from strong acids and oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (IR / NMR)	Conforms to structure
Assay (HPLC)	> 97% (Radiochemical Purity)
Specific Activity	Customizable (e.g., 20-80 Ci/mmol)
Chemical Purity (HPLC)	> 98%
Solvent (if supplied as solution)	Ethanol/Water or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.