Description

n-Ethylmaleimide CAS NO 128-53-0 is a highly reactive, small-molecule alkylating agent widely used as a selective sulfhydryl group modifier. Its primary value lies in its ability to covalently bind to cysteine residues in proteins and peptides, making it an indispensable tool for biochemical research and process development. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical, and biotechnology sectors who require precise protein modification, enzyme inhibition, or cross-linking capabilities.

Application

• Biochemical Research: Selective modification of cysteine thiol groups in proteins and enzymes to study structure, function, and active sites.
• Enzyme Inhibition: Used to irreversibly inhibit ATPase activity and other enzymes dependent on sulfhydryl groups for function.
• Pharmaceutical Intermediates: Serves as a key building block in the synthesis of more complex bioactive molecules and potential drug candidates.
• Protein Cross-linking: Acts as a homobifunctional cross-linker for studying protein-protein interactions and complex formation.
• Polymer Chemistry: A monomer or modifier in the production of specialty polymers, including those with responsive or functional properties.
• Diagnostic Reagent Manufacturing: Used in the preparation of conjugated antibodies and other labeled biomolecules for diagnostic assays.

Basic Information

Product Name	n-Ethylmaleimide
CAS No.	128-53-0
Molecular Formula	C6H7NO2
Molecular Weight	125.13 g/mol
Synonyms	N-Ethylmaleimide; 1-Ethyl-1H-pyrrole-2,5-dione; Ethylmaleimide; Maleic acid N-ethylimide; NEM; 2,5-Pyrrolidinedione, 1-ethyl-; NSC 11808; Ethyl maleimide
EINECS	204-896-7

Quality Control

Our n-Ethylmaleimide is produced and tested under a stringent quality management system to ensure batch-to-batch consistency and high purity for critical research and industrial applications. We provide comprehensive analytical data, including purity by HPLC, identity confirmation (IR, NMR), and tests for specific impurities. A Certificate of Analysis (COA) is supplied with each batch, detailing all specifications and results. Our quality commitment aligns with the standards expected in pharmaceutical and high-purity chemical supply chains.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at room temperature (typically 15-25°C). Keep away from incompatible materials such as strong bases and reducing agents. For long-term storage under inert atmosphere, please contact our technical team for specific recommendations.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Melting Point	43 - 46 °C
Loss on Drying	≤ 0.5 %
Residue on Ignition	≤ 0.1 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.