Description

Aspartic Acid-β-4-Nitroanilide is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the sensitive and specific detection of protease activity, particularly for enzymes like trypsin and other serine proteases. It serves as an essential reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors, where precise enzyme kinetics and activity measurements are paramount.

Application

• Protease Activity Assays: Primary substrate for the colorimetric determination of trypsin, chymotrypsin, and related serine protease activities.
• Diagnostic Reagent Manufacturing: Key component in the formulation of diagnostic kits for pancreatic function and other clinical tests.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and mechanistic investigations of proteolytic enzymes.
• Pharmaceutical Quality Control: Employed in the purity and potency testing of enzyme-based pharmaceutical products.
• Food Science & Technology: Applied in research to measure protease activity in food processing and quality assessment.

Basic Information

Product Name	Aspartic Acid-β-4-Nitroanilide
CAS No.	102-66-9
Molecular Formula	C16H16N4O6
Molecular Weight	360.32 g/mol
Synonyms	N-(4-Nitrophenyl)-L-asparagine; L-Aspartic acid 4-nitroanilide; Asp-4-NA; 4-Nitroanilide of L-Aspartic Acid; N4-(4-Nitrobenzoyl)-L-2,4-diaminobutanoic acid; N-(4-Nitrophenyl)-L-asparaginic acid; Substrate for Trypsin; Chromogenic Trypsin Substrate
EINECS	203-048-9

Quality Control

Our Aspartic Acid-β-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR spectroscopy, and specific tests for residual solvents and moisture to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment to guarantee traceability, consistency, and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Yellow to light brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	205 - 210 °C (dec.)
Specific Rotation [α]D20	-15° to -20° (c=1 in DMF)
Loss on Drying	≤ 1.0%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in DMF, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.