Description

Sephadex G-100 is a cross-linked dextran gel designed for gel filtration chromatography, specifically for the separation of biomolecules in the molecular weight range of 4,000 to 150,000 Daltons. Its precise bead size and pore structure are critical for achieving high-resolution separations of proteins, peptides, and polysaccharides in aqueous solutions. This product is essential for researchers and process developers in the biotechnology, pharmaceutical, and life sciences industries who require reliable, reproducible fractionation and purification of medium-to-large biomolecules. The compound is identified by CAS No. 9050-94-6.

Application

• Size-exclusion chromatography (SEC) for the purification and analysis of proteins, enzymes, and antibodies.
• Desalting and buffer exchange of biological samples in laboratory and process-scale workflows.
• Fractionation of polysaccharides, nucleic acids, and other water-soluble polymers.
• Removal of low molecular weight contaminants or additives from protein solutions.
• Molecular weight estimation of unknown proteins via column calibration with standards.
• Initial capture and group separation steps in multi-step downstream bioprocessing.
• Support for teaching and method development in academic and industrial research laboratories.

Basic Information

Product Name	Sephadex G-100
CAS No.	9050-94-6
Molecular Formula	(C6H10O5)n
Molecular Weight	High polymer (varies)
Synonyms	Dextran gel G-100; Sephadex Gel Filtration Medium G-100; Cross-linked Dextran G-100; Gel Filtration Chromatography Beads G-100; Dextran cross-linked with epichlorohydrin; Gel Chromatography Media G-100; Size Exclusion Chromatography Media G-100
EINECS	Contact for details

Quality Control

Our Sephadex G-100 is manufactured under a rigorous quality management system. Each batch is tested to ensure consistent performance characteristics, including bead size distribution, exclusion limits, and swelling properties. While not typically compendial, production can adhere to principles of GMP for critical applications. A comprehensive Certificate of Analysis (COA) detailing physical and functional specifications is provided with every shipment to guarantee traceability and performance.

Storage

Preserve in a tightly closed container, protected from light. This material is hygroscopic (moisture-sensitive). Store in a cool, dry place at a controlled room temperature (15-25°C). Ensure the original container is sealed after each use to prevent moisture uptake, which can affect swelling volume and chromatographic performance.

Specification

Item	Specification
Appearance	White to off-white free-flowing beads
Particle Size (dry)	40 - 120 μm
Exclusion Limit (Globular Proteins)	~150,000 Daltons
Fractionation Range (Globular Proteins)	4,000 - 150,000 Daltons
Bed Volume (swollen in water)	15 - 20 mL/g dry gel
Water Regain	~10 g/g dry gel
pH Stability (long-term)	2 - 13
Operating pH Range	2 - 10
Maximum Linear Flow Rate	10 - 15 cm/h
Conductivity	< 20 μS/cm (in water)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.