Description

Tris(Hydroxymethyl)Aminomethane, widely known as Tris base, is a high-purity organic compound essential for creating stable pH environments in biochemical and molecular biology research. Its primary value lies in its excellent buffering capacity in the physiological pH range (7.0-9.0), making it a cornerstone reagent for numerous scientific and industrial processes. This product is critically needed by manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors, as well as by research institutions for applications requiring precise and reliable buffering systems.

Application

• Biochemical & Molecular Biology Buffers: Primary component of Tris-HCl and Tris-EDTA (TE) buffers for nucleic acid (DNA/RNA) electrophoresis, extraction, and purification.
• Pharmaceutical Formulation: Used as an excipient and buffering agent in parenteral formulations, vaccines, and topical products to maintain stability and efficacy.
• Protein Research & Analysis: Key buffer for polyacrylamide gel electrophoresis (PAGE), protein crystallization, and enzyme activity assays.
• Diagnostic Reagents: Serves as a buffer base in clinical diagnostic kits and reagents for consistent and accurate test results.
• Industrial Processes: Employed in surfactant synthesis, epoxy resin curing agents, and as a chemical intermediate in organic synthesis.
• Cell Culture Media: Used to prepare buffered salt solutions and media components to maintain physiological pH for cell growth.

Basic Information

Product Name	Tris(Hydroxymethyl)Aminomethane
CAS No.	77-86-1
Molecular Formula	C4H11NO3
Molecular Weight	121.14 g/mol
Synonyms	Tris base; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; Trometamol; THAM; Tris Buffer; Trisamine; Tromethamine; Trizma® Base
EINECS	201-064-4

Quality Control

Our Tris(Hydroxymethyl)Aminomethane is manufactured under a strict quality management system. It is available in various grades, including high-purity Biotechnology Grade and Pharmaceutical Grade (USP/EP), meeting stringent specifications for critical applications. Every batch undergoes comprehensive analytical testing, including assay, impurity profiling, and endotoxin testing where required. Certificates of Analysis (COA) are provided to ensure full traceability and compliance with your project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); keep the container tightly sealed to prevent absorption of atmospheric moisture and carbon dioxide.

Specification

Item	Specification
Appearance	White crystalline powder
Identification (IR)	Conforms
Assay (Titration)	≥ 99.0 %
pH (5% in water, 25°C)	10.0 - 11.5
Solubility (in water)	Clear, colorless solution
Loss on Drying	≤ 0.5 %
Residue on Ignition	≤ 0.1 %
Heavy Metals (as Pb)	≤ 10 ppm
Chloride (Cl)	≤ 50 ppm
Sulfate (SO4)	≤ 50 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.