Description

Hepes Sodium Salt CAS NO 75277-39-3 is the sodium salt form of the widely used zwitterionic organic chemical buffering agent, HEPES. This compound is essential for maintaining stable pH in a variety of sensitive biochemical and cell culture systems where traditional buffers may interfere. It is a critical reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries who require high-purity, reliable buffering components.

Application

• Cell Culture Media: As a primary buffering agent in mammalian and bacterial cell culture to maintain physiological pH (typically 7.2-7.5) in open systems with CO2.
• Protein Biochemistry & Enzymology: For stabilizing pH during protein purification, crystallization, enzymatic assays, and electrophoresis where minimal metal ion chelation is required.
• Molecular Biology Reagents: A key component in PCR buffers, nucleic acid hybridization solutions, and other molecular biology kits to ensure optimal reaction conditions.
• Pharmaceutical Formulations: Used in the development and manufacturing of biologics, vaccines, and other therapeutic products as an excipient to control pH.
• Diagnostic Kits & Reagents: Provides consistent pH environments in clinical diagnostic assays, including immunoassays and blood gas analyzers.
• In Vitro Fertilization (IVF) Media: Employed in embryo culture media due to its excellent biocompatibility and ability to maintain pH outside a CO2 incubator.

Basic Information

Product Name	Hepes Sodium Salt
CAS No.	75277-39-3
Molecular Formula	C8H17N2NaO4S
Molecular Weight	260.28 g/mol
Synonyms	HEPES sodium salt; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; Sodium HEPES; HEPES Na; 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt; EPPS sodium salt (common misnomer); Good's Buffer sodium salt
EINECS	Contact for details

Quality Control

Our Hepes Sodium Salt is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing to ensure it meets high-purity standards suitable for research and production applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided and available upon request, supporting compliance with ISO and GMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); keep the original container tightly sealed to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥98.0%
pH (1% Solution)	7.0 - 8.0
Loss on Drying	≤1.0%
Heavy Metals (as Pb)	≤10 ppm
Endotoxin (LAL)	<1.0 EU/mg (for applicable grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.