Description

HEPES (CAS NO 7365-45-9) is a zwitterionic organic chemical buffering agent renowned for its exceptional stability and minimal interference with biological systems. It is critical for maintaining precise pH control in a wide range of biochemical and cell culture applications where traditional buffers fail. This high-purity product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries who require reliable, consistent performance in sensitive environments.

Application

• Cell Culture Media: A primary buffering component for mammalian and bacterial cell cultures, providing stable pH in open systems with minimal toxicity.
• Protein Biochemistry & Enzymology: Used in enzyme assays, protein purification, and crystallization where metal ion chelation and minimal reactivity are paramount.
• Molecular Biology: Employed as a buffer in PCR, electrophoresis, and nucleic acid hybridization procedures.
• Diagnostic Reagents: Formulated into buffers for clinical diagnostic kits and immunoassays to ensure assay consistency and accuracy.
• Pharmaceutical Formulations: Serves as an excipient buffer in certain parenteral and ophthalmic drug products.
• Microscopy & Imaging: Provides a stable physiological pH environment for live-cell imaging and fluorescence microscopy studies.

Basic Information

Product Name	HEPES
CAS No.	7365-45-9
Molecular Formula	C8H18N2O4S
Molecular Weight	238.30 g/mol
Synonyms	4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid); EPPS; HEPES buffer; HEPES free acid; 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
EINECS	230-907-9

Quality Control

Our HEPES is manufactured under a strict quality management system. Each batch is subjected to comprehensive analytical testing, including identification, assay, and impurity profiling, to ensure it meets high-purity standards suitable for research and industrial use. Certificates of Analysis (COA) with detailed specifications are provided for every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at room temperature (15-25°C). The product is hygroscopic; keep the container tightly sealed to minimize moisture absorption.

Specification

Item	Specification
Appearance	White crystalline powder
Identification (IR)	Conforms
Assay (Titration)	≥ 99.0%
pH (1M aqueous solution)	7.0 - 8.0
UV Absorbance (1M, 260 nm)	≤ 0.05
UV Absorbance (1M, 280 nm)	≤ 0.05
Heavy Metals (as Pb)	≤ 10 ppm
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.