Description

Tris(Hydroxymethyl)Aminomethane Acetate Salt CAS NO 6850-28-8 is a high-purity organic salt widely used as a buffering agent in biochemical and pharmaceutical applications. Its primary value lies in providing a stable pH environment critical for enzymatic reactions, protein purification, and cell culture media formulation. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries who require reliable, high-quality buffer components for sensitive processes.

Application

• Biochemical Research Buffer: Used in electrophoresis (e.g., TAE buffer for agarose gel electrophoresis), enzyme assays, and protein crystallization.
• Pharmaceutical Formulations: Serves as an excipient and buffering agent in parenteral solutions, ophthalmic preparations, and topical products.
• Molecular Biology: A key component in nucleic acid purification kits, PCR master mixes, and DNA/RNA hybridization buffers.
• Cell Culture Media: Provides pH stability in mammalian and bacterial cell culture systems and fermentation processes.
• Diagnostic Reagents: Utilized in clinical chemistry analyzers and as a buffer in immunoassay kits and diagnostic strips.
• Chromatography: Acts as a mobile phase modifier and buffer in ion-exchange and affinity chromatography for biomolecule separation.

Basic Information

Product Name	Tris(Hydroxymethyl)Aminomethane Acetate Salt
CAS No.	6850-28-8
Molecular Formula	C5H13NO5
Molecular Weight	167.16 g/mol
Synonyms	Tris Acetate; Tromethamine Acetate; 2-Amino-2-(hydroxymethyl)-1,3-propanediol acetate; TRIS Acetate Salt; Tris base acetate; Trizma® Acetate; Tris(hydroxymethyl)methylammonium acetate; Amino Tris(hydroxymethyl)methane Acetate
EINECS	229-939-6

Quality Control

Our Tris(Hydroxymethyl)Aminomethane Acetate Salt is manufactured under a strict quality management system. Each batch is tested to ensure it meets high-purity standards suitable for research and industrial applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as assay, pH, and impurity profiles. The product can be supplied to meet various grade requirements, including reagent and biotech grades.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect quality and performance.

Specification

Item	Specification
Appearance	White crystalline powder
Identification (IR)	Conforms
Assay (Titration)	≥ 99.0 %
pH (1% Solution)	6.5 - 7.5
Loss on Drying	≤ 1.0 %
Residue on Ignition	≤ 0.1 %
Heavy Metals (as Pb)	≤ 10 ppm
Chloride (Cl)	≤ 0.005 %
Sulfate (SO4)	≤ 0.005 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.