Description

Hepps CAS NO 16052-06-5 is a high-purity biochemical buffer agent, chemically known as 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid. This compound is essential for maintaining stable pH conditions in a wide range of sensitive biochemical and biological research applications. It is a critical reagent for scientists and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries who require reliable, non-interfering buffering systems.

Application

• Biochemical Research Buffer: A zwitterionic Good's buffer used to maintain physiological pH (7.0-8.5) in cell culture media, enzyme assays, and protein purification protocols.
• Pharmaceutical Formulation: Employed as a buffering agent in the development and manufacturing of biologics, vaccines, and other sensitive therapeutic products.
• Molecular Biology: Provides stable pH for PCR, electrophoresis, nucleic acid hybridization, and other critical molecular techniques.
• Diagnostic Reagents: Used as a key component in the formulation of clinical diagnostic kits and assays to ensure accuracy and reproducibility.
• Tissue Culture: An effective buffer for mammalian and bacterial cell culture systems, minimizing osmotic stress and metal ion chelation.
• Chromatography: Serves as a mobile phase modifier or equilibration buffer in various chromatographic separations, including HPLC and FPLC.

Basic Information

Product Name	Hepps
CAS No.	16052-06-5
Molecular Formula	C9H20N2O4S
Molecular Weight	252.33 g/mol
Synonyms	HEPPS; EPPS; 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid; 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid); Good's Buffer HEPPS; Acid, 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic
EINECS	240-198-4

Quality Control

Our Hepps is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure it meets high-purity standards suitable for research and pharmaceutical applications. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming specifications for identity, purity, and impurity profiles. We can supply material compliant with various pharmacopeial standards upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed to prevent absorption of atmospheric moisture, which can affect purity and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (Titration)	≥ 99.0%
pH (1% Solution)	7.0 - 8.5
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm
UV Absorbance (260 nm)	≤ 0.05
UV Absorbance (280 nm)	≤ 0.05

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.