Description

Tris Hydrochloride is a high-purity biochemical reagent widely used as a buffering agent in molecular biology and biochemistry. Its primary value lies in providing a stable and consistent pH environment critical for enzymatic reactions, protein purification, and nucleic acid electrophoresis. This product is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries who require reliable, high-quality buffer components for sensitive applications. Tris Hydrochloride CAS NO 1185-53-1 is available in various grades to meet specific research and production needs.

Application

• Molecular Biology Buffers: A key component in TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA) buffers for agarose gel electrophoresis of DNA and RNA.
• Protein Biochemistry: Used in buffer systems for protein purification, cell lysis, and chromatography (e.g., size-exclusion, ion-exchange).
• Diagnostic Reagent Formulation: Serves as a buffering agent in the formulation of clinical diagnostic kits and assays.
• Cell Culture Media: Employed to maintain physiological pH in certain cell culture and tissue engineering applications.
• Pharmaceutical Processing: Utilized as an excipient or buffering agent in the development and manufacturing of biopharmaceuticals.
• Biochemical Research: A fundamental reagent for in-vitro enzymatic studies, PCR master mixes, and various biochemical assays requiring pH control.

Basic Information

Product Name	Tris Hydrochloride
CAS No.	1185-53-1
Molecular Formula	C4H11NO3 • HCl
Molecular Weight	157.60 g/mol
Synonyms	Tris HCl; Tromethamine Hydrochloride; 2-Amino-2-(hydroxymethyl)-1,3-propanediol Hydrochloride; THAM Hydrochloride; Tris(hydroxymethyl)aminomethane Hydrochloride; Tris Buffer HCl; TRIS-HCl; Trometamol Hydrochloride
EINECS	214-684-6

Quality Control

Our Tris Hydrochloride is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing to ensure it meets high-purity standards suitable for life science applications. Certificates of Analysis (COA) detailing parameters such as assay, pH, clarity, and endotoxin levels (where applicable) are provided with every shipment. We can supply grades compliant with various pharmacopeial monographs upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect purity and performance.

Specification

Item	Specification
Appearance	White crystalline powder
Identification (IR)	Conforms
Assay (Titration)	≥ 99.0 %
pH (1M in H2O)	3.5 - 5.0
Clarity of Solution	Clear, colorless
Heavy Metals (as Pb)	≤ 10 ppm
Loss on Drying	≤ 0.5 %
Residue on Ignition	≤ 0.1 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.