Description

1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-, chloride (1:1) is a high-purity, functionalized cyanine dye derivative designed for advanced material science and life science applications. This compound is valued for its excellent photosensitivity and reactive N-hydroxysuccinimide (NHS) ester group, enabling stable covalent conjugation to biomolecules and polymers. It is primarily sought by researchers and manufacturers in the fields of bioconjugation, fluorescent labeling, and the development of optical materials and sensors.

Application

• Bioconjugation and Fluorescent Probe Synthesis: The NHS ester moiety allows for efficient, stable labeling of proteins, antibodies, peptides, and oligonucleotides for bioimaging and diagnostic assays.
• Advanced Material Development: Used in the synthesis of functional dyes for optical data storage, nonlinear optical (NLO) materials, and light-harvesting arrays.
• Sensor Fabrication: Serves as a key component in the development of fluorescent chemosensors for detecting ions, small molecules, or changes in the local environment.
• Polymer and Surface Modification: Employed to covalently attach fluorescent tags to polymers, nanoparticles, or solid surfaces for tracking and characterization studies.
• Photodynamic Therapy (PDT) Research: Investigated as a potential photosensitizer precursor due to its benz[e]indolium core structure and strong absorption properties.
• Academic and Industrial R&D: A critical reagent for synthetic chemistry and photophysical research in university and corporate laboratories.

Basic Information

Product Name	1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-, chloride (1:1)
CAS No.	1469277-96-0
Molecular Formula	C43H48ClN3O4
Molecular Weight	706.31 g/mol
Synonyms	Cy5 NHS ester; Sulfo-Cy5 NHS ester analog (non-sulfonated); 1,1,3-Trimethyl-2-[5-(1,1,3-trimethyl-1,3-dihydro-2H-benz[e]indol-2-ylidene)penta-1,3-dien-1-yl]-3-[6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl]-1H-benz[e]indol-3-ium chloride; Cyanine5 NHS ester; NHS-Cy5; Pentamethine cyanine dye NHS ester; Benz[e]indolium cyanine dye active ester
EINECS	Contact for details

Quality Control

Our production of this specialty dye adheres to strict in-process controls to ensure batch-to-batch consistency and high chemical purity. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the integrity of the reactive NHS ester group. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed container with silica gel) due to its hygroscopic and light-sensitive nature. For long-term stability, store under an inert atmosphere such as argon or nitrogen. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF, acetonitrile
Extinction Coefficient	Contact for details
Functional Group Integrity (NHS Ester)	≥ 90% by assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.