Description

4-Chloro-7-Nitrobenzofurazan (CAS NO 10199-89-0) is a specialized heterocyclic organic compound that serves as a critical derivatization reagent and fluorescent probe. Its primary value lies in its high reactivity with amines and thiols, forming stable, highly fluorescent adducts suitable for sensitive detection and quantification. This makes it an indispensable tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and analytical chemistry sectors who require precise labeling and detection of biomolecules.

Application

• Derivatization Agent for HPLC/LC-MS: Used to label primary and secondary amines, amino acids, and peptides to enhance their detection sensitivity and chromatographic properties.
• Fluorescent Labeling of Biomolecules: Acts as a fluorogenic tag for proteins, antibodies, and other macromolecules in biochemical assays and imaging studies.
• Proteomics and Amino Acid Analysis: Employed in pre-column derivatization for the accurate analysis of amino acid composition in proteins and hydrolysates.
• Detection of Thiol Groups: Reacts selectively with sulfhydryl (-SH) groups in cysteine residues and other thiol-containing compounds for quantification.
• Research Chemical: Serves as a key synthetic intermediate and building block in organic chemistry research for constructing complex benzofurazan-based structures.
• Quality Control in Pharmaceutical Manufacturing: Utilized in analytical methods for the trace analysis of amines and related impurities in drug substances and products.

Basic Information

Product Name	4-Chloro-7-Nitrobenzofurazan
CAS No.	10199-89-0
Molecular Formula	C₆H₂ClN₃O₃
Molecular Weight	199.55 g/mol
Synonyms	4-Chloro-7-nitro-2,1,3-benzoxadiazole; NBD-Chloride; 4-Chloro-7-nitrobenz-2-oxa-1,3-diazole; 7-Nitro-4-chlorobenzofurazan; 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole; NBD-Cl; 4-Chloro-7-nitro-2,1,3-benzoxadiazole; 10199-89-0
EINECS	233-476-8

Quality Control

Every batch of 4-Chloro-7-Nitrobenzofurazan is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and consistent performance. Our quality system ensures compliance with relevant standards for research and analytical applications. A comprehensive Certificate of Analysis (COA), detailing purity, identity, and impurity profiles as determined by techniques such as HPLC, NMR, and IR, is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at a controlled room temperature (15-25°C). This compound is light-sensitive (store away from light) and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	97 - 102 °C
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.