Description

n-2-4-Dnp-Hydroxy-L-Proline Crystalline is a high-purity, crystalline derivative of the amino acid L-proline, specifically modified with a 2,4-dinitrophenyl (DNP) group. This compound is a critical reagent and analytical standard used in advanced biochemical research and quality control processes. It is primarily utilized by research institutions, pharmaceutical development laboratories, and analytical service providers for applications requiring precise molecular identification and quantification. The product is supplied as a crystalline solid to ensure stability and ease of handling.

Application

• Biochemical Research: Used as a key intermediate or reference standard in studies involving amino acid metabolism, protein structure, and enzyme kinetics.
• Pharmaceutical Analysis: Serves as a critical standard in High-Performance Liquid Chromatography (HPLC) and mass spectrometry for the identification and purity assessment of proline-related compounds in drug substances.
• Proteomics and Peptide Chemistry: Employed in the derivatization and analysis of peptides and proteins to facilitate separation, detection, and structural elucidation.
• Quality Control (QC) Laboratories: Essential for method development, validation, and routine testing in QC labs to ensure the accuracy of analytical procedures for amino acids and related molecules.
• Academic Research: Utilized in university and institutional labs for teaching and investigative projects in organic chemistry, biochemistry, and analytical chemistry.
• Diagnostic Reagent Manufacturing: Can be incorporated into the production of specialized diagnostic kits and reagents for clinical testing.

Basic Information

Product Name	n-2-4-Dnp-Hydroxy-L-Proline Crystalline
CAS No.	16220-71-6
Molecular Formula	C₁₁H₁₁N₃O₇
Molecular Weight	297.23 g/mol
Synonyms	N-(2,4-Dinitrophenyl)-4-hydroxy-L-proline; 4-Hydroxy-L-proline N-(2,4-dinitrophenyl) derivative; L-4-Hydroxyproline 2,4-dinitrophenyl derivative; DNP-Hyp-OH; (2S,4R)-1-(2,4-Dinitrophenyl)-4-hydroxypyrrolidine-2-carboxylic acid; Hydroxyproline DNP derivative; 4-Hydroxy-L-proline DNP; N-2,4-DNP-trans-4-hydroxy-L-proline
EINECS	Contact for details

Quality Control

Our n-2-4-Dnp-Hydroxy-L-Proline Crystalline is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation, to ensure it meets the high standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and physical characteristics is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Melting Point	Specification available upon request
Loss on Drying	≤ 0.5%
Heavy Metals	< 20 ppm
Residual Solvents	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.