Description

Aminoallyl-dUTP Sodium Salt is a chemically modified nucleotide triphosphate essential for advanced molecular labeling and detection techniques. Its core value lies in providing a primary amine handle for the covalent attachment of various fluorescent dyes, biotin, or other reporter molecules, enabling the creation of highly sensitive, non-radioactive probes. This modified nucleotide is a critical reagent for researchers and developers in molecular biology, genomics, and diagnostic assay development who require efficient and versatile nucleic acid labeling.

Application

• Fluorescent DNA/RNA Probe Synthesis: Enzymatic incorporation into nucleic acids for subsequent conjugation with amine-reactive fluorophores (e.g., Cy3, Cy5, Alexa Fluor dyes) for FISH, microarray analysis, and real-time PCR.
• Biotinylated Probe Generation: Creation of probes for detection via streptavidin-enzyme conjugates in blotting, in situ hybridization, and capture-based assays.
• Next-Generation Sequencing (NGS) Library Preparation: Used in certain protocols for labeling and amplifying cDNA or genomic DNA libraries.
• DNA Microarray Fabrication: Labeling of target DNA or cDNA for hybridization onto array chips for gene expression profiling.
• Single Nucleotide Polymorphism (SNP) Detection: Enables the production of labeled primers or probes for high-throughput genotyping platforms.
• Diagnostic Kit Development: A key component in the manufacture of labeled nucleic acid components for clinical and research diagnostic tests.

Basic Information

Product Name	Aminoallyl-dUTP Sodium Salt
CAS No.	936327-10-5
Molecular Formula	C14H20N4O13P3Nan (n~3)
Molecular Weight	Approx. 663.1 (Free acid)
Synonyms	5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate sodium salt; AA-dUTP Na Salt; Aminoallyl-2'-deoxyuridine-5'-triphosphate; 5-(3-Aminoallyl)-dUTP; 5-(3-Aminoprop-2-en-1-yl)-2'-deoxyuridine-5'-triphosphate sodium salt; Allylamine-dUTP; Aminoallyl labeled dUTP
EINECS	Contact for details

Quality Control

Our Aminoallyl-dUTP Sodium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and functional validation in enzymatic incorporation assays, to ensure it meets the high standards required for sensitive life science research. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	Suitable for enzymatic incorporation
Water Content (KF)	≤ 5%
Solubility	Soluble in water, TE buffer (pH 8.0)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.