Description

Apurinic Acid is a high-purity biochemical reagent derived from DNA, characterized by the absence of purine bases. This compound is essential for advanced molecular biology research and diagnostic applications, serving as a critical substrate and standard. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic kit manufacturers requiring precise and reliable nucleic acid derivatives. Our supply ensures consistent quality and stability for sensitive experimental protocols.

Application

• Molecular Biology Research: Used as a substrate in enzymatic studies, particularly for apurinic/apyrimidinic (AP) endonucleases and DNA repair pathway analysis.
• Diagnostic Assay Development: Serves as a critical component and standard in the development of kits for detecting DNA damage and oxidative stress.
• Biochemical Standard: Acts as a reference material for calibrating instruments and validating methods in analytical laboratories.
• Pharmaceutical Testing: Employed in quality control and research labs to study the genotoxic effects and DNA-interaction mechanisms of new drug candidates.
• Educational & Teaching Tools: Provides a well-defined compound for university-level courses in biochemistry, genetics, and molecular biology.

Basic Information

Product Name	Apurinic Acid
CAS No.	11002-22-5
Molecular Formula	Typically represented as (C9H11N2O8P)n
Molecular Weight	Variable (Polymeric)
Synonyms	Apurinic Acid; Depurinated DNA; AP DNA; AP Site-containing DNA; DNA, apurinic; Depurinic Acid; Abasic DNA; 2'-Deoxyribose-containing DNA.
EINECS	Contact for details

Quality Control

Our Apurinic Acid is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and consistency. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key parameters such as assay, absorbance ratios, and absence of contaminating nucleases. We adhere to cGMP principles for manufacturing, ensuring reliability for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation. For extended storage under inert atmosphere, please contact our technical team for specific recommendations.

Specification

Item	Specification
Appearance	White to off-white fibrous solid or lyophilized powder
Identification (IR)	Conforms to structure
Assay (UV-Vis)	≥ 95% (as apurinic sites)
A260/A280 Ratio	1.8 - 2.0
Protein Contamination	≤ 0.1% (Bradford Assay)
Nuclease Activity	Not detected
Solubility	Soluble in aqueous buffers (e.g., Tris-EDTA), slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.