Description

5-(Dansylglycyl-6-Aminohexanoylaminoprop-1-Enyl)-2'-Deoxyuridine 5'-Triphosphate is a specialized, fluorescently labeled nucleotide analog designed for advanced molecular biology and diagnostic applications. This compound matters because it provides a critical tool for sensitive detection and analysis in nucleic acid sequencing, hybridization assays, and enzymatic studies. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in genomics, molecular diagnostics, and probe development.

Application

• Fluorescent DNA Sequencing: Used as a chain-terminating substrate in Sanger sequencing and next-generation sequencing platforms for high-sensitivity detection.
• Nucleic Acid Labeling & Probe Synthesis: Essential for enzymatically incorporating a dansyl fluorophore into oligonucleotides for FISH (Fluorescence In Situ Hybridization) and microarray applications.
• Enzyme Kinetics & Mechanistic Studies: Serves as a substrate to study the activity and specificity of DNA polymerases, reverse transcriptases, and other nucleotide-processing enzymes.
• Diagnostic Assay Development: Incorporated into probes for real-time PCR and other amplification-based diagnostic tests to enhance signal generation.
• Single-Molecule Detection: Utilized in advanced microscopy and biosensing techniques that require high photon yield and photostability from incorporated nucleotides.
• Apoptosis Detection (TUNEL Assay): Can be used in modified TUNEL assays for labeling DNA strand breaks in apoptotic cells with a fluorescent tag.

Basic Information

Product Name	5-(Dansylglycyl-6-Aminohexanoylaminoprop-1-Enyl)-2'-Deoxyuridine 5'-Triphosphate
CAS No.	109921-29-1
Molecular Formula	C₃₆H₄₈N₇O₁₉P₃
Molecular Weight	999.74 g/mol
Synonyms	Dansyl-dUTP; 5-([N-((5-Dimethylaminonaphthalene-1-sulfonyl)glycyl)-6-aminohexanoyl]aminoprop-1-enyl)-2'-deoxyuridine-5'-triphosphate; Dns-dUTP; Fluorescent dUTP; 5-(Dansyl-ε-aminocaproyl-3-aminoprop-1-enyl)-dUTP; 5-(Dansyl-6-aminohexanoylaminopropenyl)-2'-deoxyuridine 5'-triphosphate; 5-[3-[6-[[2-[(5-Dimethylaminonaphthalen-1-yl)sulfonylamino]acetyl]amino]hexanoylamino]prop-1-en-1-yl]-2'-deoxyuridine 5'-(trihydrogen triphosphate)
EINECS	Contact for details

Quality Control

This high-purity biochemical is manufactured under strict quality control protocols to ensure batch-to-batch consistency and performance in sensitive applications. Each lot is characterized by HPLC for purity and identity, with specific testing for enzymatic incorporation efficiency. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance profiles, and functional performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized solid or solution
Identification (HPLC)	Conforms to reference standard
Identification (UV-Vis)	Characteristic absorption spectrum
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~4,300 M⁻¹cm⁻¹ at 340 nm (in H₂O)
Solubility	Soluble in water, TE buffer, or DMSO
Enzymatic Incorporation	Suitable for use with common DNA polymerases

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.