Description

Fluorescein n-Acetylimidazole is a specialized, reactive derivative of fluorescein, designed for advanced conjugation and labeling applications. This compound matters for its role as a key intermediate in the synthesis of highly sensitive fluorescent probes and diagnostic reagents. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for developing assays, bioimaging agents, and novel therapeutic conjugates.

Application

• Synthesis of Fluorescent Probes: Acts as a critical intermediate for creating site-specific fluorescent labels for proteins, antibodies, and other biomolecules.
• Diagnostic Reagent Manufacturing: Used in the production of reagents for fluorescence-based immunoassays (e.g., ELISA, lateral flow tests) and histochemical staining.
• Bioimaging Agent Development: Employed in research to develop novel contrast agents for cellular and molecular imaging applications.
• Pharmaceutical Research: Serves as a building block for drug discovery programs, particularly in creating drug-fluorescent dye conjugates for pharmacokinetic and biodistribution studies.
• Bioconjugation Chemistry: Utilized for attaching fluorescein moieties to polymers, nanoparticles, and surfaces under controlled conditions.
• Quality Control in Biologics: Applied in the development of internal standards and calibration materials for analytical methods.

Basic Information

Product Name	Fluorescein n-Acetylimidazole
CAS No.	97068-28-5
Molecular Formula	C25H16N2O6
Molecular Weight	440.41 g/mol
Synonyms	Fluorescein N-Acetylimidazole; 2-[6-(2,4-Dihydroxy-3-acetylimidazol-1-yl)benzoyl]benzoic Acid; 3',6'-Dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1'-yl N-Acetylimidazole; Fluorescein Imidazolide; Fluorescein Acetylimidazolide; Reactive Fluorescein Derivative; FITC Precursor
EINECS	Contact for details

Quality Control

Our Fluorescein n-Acetylimidazole is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and manufacturing. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and handled under appropriate conditions to maintain stability and reactivity.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 97.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in common organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.