Description

2',3'-Dideoxyadenosine-5'-Triphosphate Lithium Salt is a chemically modified nucleotide analog where the ribose sugar lacks hydroxyl groups at the 2' and 3' positions, rendering it a chain-terminating substrate in enzymatic synthesis. This property is critical for applications requiring controlled termination of nucleic acid polymerization, ensuring precise and reliable results in molecular biology workflows. It is an essential reagent for researchers and manufacturers in the fields of molecular diagnostics, next-generation sequencing (NGS), and enzymology who require high-purity, bio-active nucleotide derivatives.

Application

• DNA Sequencing: Used as a chain-terminating dideoxynucleotide (ddATP) in Sanger sequencing methodologies.
• Next-Generation Sequencing (NGS): Serves as a reversible terminator in certain sequencing-by-synthesis platforms.
• Enzymatic Synthesis: Acts as a substrate for DNA polymerases and reverse transcriptases in controlled synthesis experiments.
• Molecular Diagnostics: Employed in assay development for pathogen detection and genetic testing where precise chain termination is required.
• Biochemical Research: Utilized in studies of enzyme kinetics, mechanism, and inhibition for nucleic acid-processing enzymes.
• Antiviral Research: Investigated as a potential metabolite or analog in the study of nucleoside-based antiviral agents.

Basic Information

Product Name	2',3'-Dideoxyadenosine-5'-Triphosphate Lithium Salt
CAS No.	93939-70-9
Molecular Formula	C10H13Li4N5O12P3 (or commonly represented as C10H12N5O12P3 · xLi)
Molecular Weight	Approx. 507.1 (Free acid), Contact for exact salt form weight.
Synonyms	ddATP Lithium Salt; 2',3'-Dideoxyadenosine Triphosphate Lithium Salt; Dideoxyadenosine-5'-triphosphate Li salt; Adenosine, 2',3'-dideoxy-, 5'-(tetrahydrogen triphosphate), lithium salt; 2',3'-ddATP-Li; Chain-terminating ddATP; Li-ddATP; Dideoxy-ATP lithium salt
EINECS	Contact for details.

Quality Control

Our 2',3'-Dideoxyadenosine-5'-Triphosphate Lithium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for sensitive molecular biology applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90% (as active triphosphate)
Water Content (KF)	≤ 5.0%
pH (in solution)	6.0 - 8.0
Endotoxin	< 10 EU/mg (for molecular biology grade)
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.