Description

Lipopolysaccharides (LPS) from Escherichia coli are a critical class of endotoxins and a major component of the outer membrane of Gram-negative bacteria. These high-purity biochemicals are essential for research and development in immunology, vaccine development, and quality control testing. They are primarily utilized by pharmaceutical manufacturers, biotechnology companies, and academic research institutions for applications such as immune response stimulation, pyrogen testing, and the study of sepsis and inflammatory pathways.

Application

• Immunology and Vaccine Research: Used as a potent stimulator of the innate immune system to study inflammatory responses, cytokine production, and adjuvant activity.
• Pyrogen and Endotoxin Testing: Serves as a standard reference material in the Limulus Amebocyte Lysate (LAL) test to validate the absence of bacterial endotoxins in pharmaceuticals, medical devices, and parenteral solutions.
• Sepsis and Shock Models: Employed in preclinical research to induce and study systemic inflammatory response syndrome (SIRS) and septic shock in animal models.
• Cell Culture and Signaling Studies: Activates Toll-like receptor 4 (TLR4) pathways in various cell lines (e.g., macrophages, dendritic cells) to investigate intracellular signaling cascades.
• Quality Control in Biomanufacturing: Acts as a challenge agent to validate the capacity of purification processes to remove endotoxins from biologic drugs and recombinant proteins.
• Material Science and Biosensor Development: Used to functionalize surfaces for studying host-pathogen interactions and developing diagnostic sensors.

Basic Information

Product Name	Lipopolysaccharides, Escherichia Coli
CAS No.	93572-42-0
Molecular Formula	Variable polymeric structure (Lipid A + Core oligosaccharide + O-antigen)
Molecular Weight	Variable (typically 10-20 kDa)
Synonyms	E. coli LPS; Endotoxin, E. coli; Bacterial Lipopolysaccharide; O111:B4 LPS (common serotype); Lipoglycan; Pyrogen; Boivin Antigen; LOS (Lipooligosaccharide); S-LPS (Smooth-form LPS)
EINECS	Contact for details

Quality Control

Our Lipopolysaccharides are produced and tested under stringent conditions to ensure high purity, biological activity, and consistency for critical research and manufacturing applications. Each batch is characterized for key parameters including endotoxin activity (in EU/mL), protein content, and nucleic acid contamination. Certificates of Analysis (COA) detailing comprehensive test results, including identity confirmation by IR spectroscopy and activity by LAL assay, are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (IR)	Conforms to reference spectrum
Endotoxin Activity (LAL)	>1 x 10^6 EU/mg (Typical)
Protein Content (Lowry)	< 1%
Nucleic Acid Content (A260)	< 2%
Loss on Drying	< 5.0%
Biological Activity	Induces TNF-α release in standard cell assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.