Description

Ancestim CAS NO 163545-26-4 is a high-purity recombinant human stem cell factor (SCF) analog, a critical cytokine for hematopoietic and stem cell research. This product is essential for supporting the proliferation, differentiation, and survival of progenitor cells in both in vitro and in vivo studies. It is primarily utilized by researchers and developers in the biotechnology and pharmaceutical industries for applications in hematopoiesis research, cell culture, and therapeutic development.

Application

• Hematopoietic Stem Cell Expansion: Used in ex vivo culture systems to amplify hematopoietic stem and progenitor cells (HSPCs) for research and clinical applications.
• Cell Culture Supplement: A key component in defined media for the maintenance and growth of primary bone marrow cells and certain cell lines.
• Research on Mast Cells: Critical for the differentiation, survival, and functional studies of mast cells derived from bone marrow or cord blood.
• Pre-Clinical Development: Employed in animal models to study the effects of SCF on hematopoiesis, tissue repair, and as part of combination cytokine therapies.
• Drug Discovery Screening: Used in assay development to identify modulators of the c-Kit receptor signaling pathway.
• Regenerative Medicine: Investigated for its potential role in supporting stem cell-based therapies for tissue engineering and repair.

Basic Information

Product Name	Ancestim
CAS No.	163545-26-4
Molecular Formula	C845H1343N223O243S9
Molecular Weight	18.8 kDa (approx.)
Synonyms	Recombinant Human Stem Cell Factor; rHuSCF; Ancestim; SCF (165 a.a.); Stem Cell Factor (human); Mast Cell Growth Factor; MGF; Kit Ligand; KL; Steel Factor; SLF; c-kit Ligand; Megakaryocyte Growth and Development Factor
EINECS	Contact for details

Quality Control

Our Ancestim is manufactured under strict cGMP guidelines to ensure the highest standards of purity, identity, and biological activity. Each lot is subjected to a comprehensive battery of tests including SDS-PAGE, HPLC for purity, endotoxin testing (LAL), and rigorous bioactivity assays. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming compliance with specified release criteria.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, keep the lyophilized powder at -20°C or below. Reconstituted solutions should be aliquoted and stored at -20°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band, ~18-20 kDa
Purity (HPLC)	≥ 95%
Biological Activity (Cell Proliferation)	ED50 ≤ 5.0 ng/mL
Endotoxin (LAL)	< 1.0 EU/µg
Protein Content (UV)	≥ 80% of label claim
Sterility (Membrane Filtration)	Sterile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.