Description

5'-o-Thymidyl 3'-o-(2'-Deoxyadenosyl) Phosphorothioate is a synthetic, non-hydrolyzable phosphorothioate-linked dinucleotide analog, a critical building block in oligonucleotide synthesis and biochemical research. This compound matters for its role in creating nuclease-resistant DNA sequences, which are essential for studying enzyme mechanisms, developing antisense oligonucleotides, and advancing therapeutic nucleic acid technologies. It is primarily needed by biotechnology and pharmaceutical R&D teams, as well as academic researchers focused on molecular biology, genetics, and the development of novel diagnostic and therapeutic agents.

Application

• Oligonucleotide Synthesis: As a key phosphorothioate monomer for the solid-phase synthesis of modified DNA strands with enhanced stability.
• Antisense & Therapeutic Research: For the development of antisense oligonucleotides and siRNA where nuclease resistance is crucial for therapeutic efficacy.
• Enzymology Studies: Used as a substrate or inhibitor to investigate the mechanisms of nucleases, polymerases, and other DNA-modifying enzymes.
• Structural Biology: Incorporated into DNA sequences for X-ray crystallography or NMR studies to understand nucleic acid-protein interactions.
• Diagnostic Probe Development: Enables the creation of stable, high-affinity hybridization probes for sensitive detection assays.
• Bioconjugation & Labeling: Serves as a starting point for the site-specific attachment of reporter molecules (e.g., fluorophores, biotin) to oligonucleotides.

Basic Information

Product Name	5'-o-Thymidyl 3'-o-(2'-Deoxyadenosyl) Phosphorothioate
CAS No.	92127-73-6
Molecular Formula	C25H31N7O12P2S
Molecular Weight	739.57 g/mol
Synonyms	d(TpsA); Thymidylyl-(3'→5')-2'-deoxyadenosine Phosphorothioate; 5'-O-Thymidylyl-(3'→5')-2'-deoxyadenosyl Phosphorothioate; d(Tp(S)A); PS-dinucleotide (T-A); Phosphorothioate-linked T-A Dimer; Non-hydrolyzable T-A Dinucleotide; d(TpsdA)
EINECS	Contact for details

Quality Control

Our 5'-o-Thymidyl 3'-o-(2'-Deoxyadenosyl) Phosphorothioate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via mass spectrometry. Certificates of Analysis (COA) detailing all specifications are available upon request, supporting compliance with internal quality standards for pharmaceutical and biotechnology research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.