Description

5-Phosphoribosyl 1-o-(2-Thiodiphosphate) is a high-purity biochemical reagent, a sulfur-containing analog of the key metabolic intermediate PRPP (5-phosphoribosyl-1-pyrophosphate). This compound is critical for researchers studying enzyme mechanisms, particularly those involved in nucleotide biosynthesis and salvage pathways where the thiodiphosphate moiety acts as a substrate or inhibitor. It is an essential tool for biochemical and pharmaceutical research, enabling detailed kinetic and structural analysis of phosphoribosyltransferases and related enzymes. The product is supplied with guaranteed purity and stability for reliable experimental results.

Application

• Enzyme Mechanism Studies: Used as a substrate analog to investigate the catalytic mechanisms of phosphoribosyltransferases (PRTases), including adenine, hypoxanthine-guanine, and orotate phosphoribosyltransferases.
• Nucleotide Metabolism Research: Serves as a key reagent in studies of de novo and salvage pathways for purine and pyrimidine nucleotide biosynthesis.
• Pharmaceutical Development: Employed in high-throughput screening and inhibitor design for enzymes targeted in anticancer, antimicrobial, and immunosuppressive therapies.
• Structural Biology: Utilized in X-ray crystallography and NMR studies to determine enzyme-ligand complex structures and understand binding interactions.
• Biochemical Assay Development: Acts as a standard or control in developing sensitive assays for enzyme activity and inhibitor potency.
• Academic & Industrial R&D: A fundamental research chemical for universities, government labs, and biotech companies exploring nucleotide-related biochemistry.

Basic Information

Product Name	5-Phosphoribosyl 1-o-(2-Thiodiphosphate)
CAS No.	91385-22-7
Molecular Formula	C5H13O13P3S
Molecular Weight	406.11 g/mol
Synonyms	PRT-S; 5-Phosphoribosyl 1-(2-Thiodiphosphate); 5-Phospho-α-D-ribofuranosyl 1-(2-thiodiphosphate); 5-O-Phosphono-α-D-ribofuranosyl 1-(2-thiodiphosphate); α-5-Phosphoribosyl 1-(2-thiodiphosphate); Thio-PRPP; 1-(2-Thiodiphosphoryl)-5-phosphoribose; P1-(5-Phospho-α-D-ribofuranosyl) P2-thiodiphosphate
EINECS	Contact for details

Quality Control

Our 5-Phosphoribosyl 1-o-(2-Thiodiphosphate) is manufactured under controlled conditions to ensure the highest standards of purity and consistency, suitable for sensitive research applications. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. We provide comprehensive Certificates of Analysis (COA) detailing all quality parameters, ensuring full traceability and compliance with research-grade specifications. Our quality system is designed to meet the exacting requirements of the global biochemical research community.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. This product is hygroscopic (moisture-sensitive); ensure the container is sealed immediately after use to minimize exposure to atmospheric humidity. For long-term stability, store under an inert atmosphere such as argon or nitrogen. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Biological Activity	Conforms (Enzymatic assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.