Description

Flurescein-Nhs Ester is a highly reactive amine-reactive fluorescent labeling reagent. This compound is critical for the covalent conjugation of fluorescent tags to proteins, peptides, antibodies, and other biomolecules containing primary amines. It is essential for researchers and manufacturers in the life sciences, diagnostics, and bioconjugation sectors who require precise and stable fluorescent labeling for applications like flow cytometry, immunoassays, and microscopy.

Application

• Bioconjugation & Probe Synthesis: Primary application for labeling antibodies, proteins, peptides, and oligonucleotides with a bright green fluorescent tag.
• Diagnostic Assay Development: Used in the manufacture of fluorescent probes and reagents for ELISA, lateral flow assays, and other immunoassays.
• Cellular Imaging & Flow Cytometry: Enables the preparation of fluorescently-labeled ligands and antibodies for cell surface staining and intracellular tracking.
• Protein & Biomolecule Analysis: Facilitates fluorescence-based detection and quantification in gel electrophoresis, blotting, and HPLC analysis.
• Drug Discovery & High-Throughput Screening (HTS): Serves as a key reagent for labeling target molecules in fluorescence-based binding and activity assays.
• Materials Science: Used to functionalize nanoparticles, polymers, and surfaces with fluorescent properties for sensing and imaging applications.

Basic Information

Item	Details
Product Name	Flurescein-Nhs Ester
CAS No.	890090-41-2
Molecular Formula	C30H17NO9
Molecular Weight	535.46 g/mol
Synonyms	5(6)-Carboxyfluorescein N-succinimidyl ester; 6-FAM, SE; 5(6)-FAM, SE; Fluorescein-5(6)-isothiocyanate isomer mixture; FITC NHS ester; 5(6)-Carboxyfluorescein succinimidyl ester; Fluorescein NHS ester; NSC 113992
EINECS	Contact for details

Quality Control

Our Flurescein-Nhs Ester is manufactured under strict quality protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation work. A Certificate of Analysis (COA) detailing purity, absorbance, and fluorescence properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~492 nm
Emission Maximum	~518 nm
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.