Description

5'-o-(4,4'-Dimethoxytrityl)-N1-Methylpseudouridine is a key protected nucleoside derivative, specifically a DMT-protected analog of the modified nucleobase N1-methylpseudouridine. This compound matters as a critical building block in the solid-phase synthesis of therapeutic oligonucleotides, particularly mRNA, where it enhances stability and reduces immunogenicity. It is essential for pharmaceutical R&D, biotechnology companies, and CDMOs developing advanced mRNA-based vaccines and therapeutics.

Application

• mRNA Synthesis: Primary use as a phosphoramidite precursor for the solid-phase synthesis of modified mRNA.
• Therapeutic Oligonucleotide Development: Incorporation into siRNA, antisense oligonucleotides, and other RNA therapeutics to improve pharmacokinetic properties.
• Vaccine Research: Critical raw material for the research and GMP production of mRNA vaccines.
• Nucleic Acid Chemistry: Used in academic and industrial research to study RNA structure, function, and stability.
• Process Development: Serves as a standard or intermediate in scaling up oligonucleotide manufacturing processes.
• Bioconjugation & Labeling: The DMT protecting group allows for selective deprotection and further functionalization of the oligonucleotide chain.

Basic Information

Product Name	5'-o-(4,4'-Dimethoxytrityl)-N1-Methylpseudouridine
CAS No.	875302-39-9
Molecular Formula	C32H34N2O7
Molecular Weight	558.62 g/mol
Synonyms	1-Methyl-5'-O-(4,4'-dimethoxytrityl)pseudouridine; N1-Methyl-5'-O-DMT-pseudouridine; 5'-O-DMT-N1-methyl-ψ; DMT-m1ψ; 1-Methylpseudouridine 5'-O-(4,4'-dimethoxytrityl) ether; ψ(m1)-5'-O-DMT; 5'-O-(Bis(4-methoxyphenyl)phenylmethoxy)-1-methylpseudouridine
EINECS	Contact for details

Quality Control

Our 5'-o-(4,4'-Dimethoxytrityl)-N1-Methylpseudouridine is manufactured under strict quality systems suitable for pharmaceutical development. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure compliance with stringent industry standards. Certificates of Analysis (COA) with detailed chromatographic data are provided for traceability and regulatory support.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.