Description

5'-o-(Dimethoxytrityl)-N4-(Diisobutylamino)Methylidene-5-(1-Propynyl)-2'-Deoxycytidine is a sophisticated, protected nucleoside derivative designed for advanced oligonucleotide synthesis. This compound matters because it serves as a critical building block for the solid-phase synthesis of modified DNA and RNA strands, enabling the creation of high-value therapeutic and diagnostic oligonucleotides. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and CROs specializing in antisense therapy, siRNA, aptamer development, and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: A key phosphoramidite precursor for automated DNA/RNA synthesizers.
• Antisense Oligonucleotide (ASO) Development: Incorporation of the 5-propynyl cytidine modification to enhance binding affinity and nuclease resistance.
• siRNA and miRNA Research: Used in the synthesis of chemically modified siRNA strands to improve stability and pharmacokinetic profiles.
• Aptamer and Diagnostic Probe Manufacturing: Enables the production of high-specificity binding agents for biosensors and assays.
• Therapeutic Nucleic Acid Production: Essential for developing advanced modalities like mRNA vaccines and gene therapies that require modified nucleosides.
• Academic and Industrial R&D: A vital reagent for studying nucleic acid structure, function, and for exploring novel genetic medicine platforms.

Basic Information

Product Name	5'-o-(Dimethoxytrityl)-N4-(Diisobutylamino)Methylidene-5-(1-Propynyl)-2'-Deoxycytidine
CAS No.	869355-42-0
Molecular Formula	C41H48N4O6
Molecular Weight	692.85 g/mol
Synonyms	5'-O-DMT-N4-(Diisobutylamino)Methylidene-5-Propynyl-dC; 5-Propynyl-dC Phosphoramidite Synthon; DMT-5-Propynyl-dC(N4-Dibutylformamidine); N4-(Diisobutylamino)methylene-5-(1-propynyl)-2'-deoxycytidine 5'-O-(4,4'-dimethoxytrityl); 5-Propynyl-2'-deoxycytidine derivative; Protected 5-Propynylcytidine; Amidite precursor for oligonucleotide synthesis
EINECS	Contact for details

Quality Control

Our production of this high-purity nucleoside derivative adheres to stringent GMP-grade principles for critical pharmaceutical intermediates. Every batch undergoes comprehensive analytical characterization, including HPLC for purity assessment and NMR for structural confirmation, to ensure consistency and reliability for sensitive synthesis workflows. Certificates of Analysis (COA) detailing purity, identity, and moisture content are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or ambient light will compromise stability and performance.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DCM, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.