Description

Polyriboadenylic Acid-Polyribo-5-Dimethylaminouridylic Acid is a synthetic, double-stranded RNA complex composed of polyadenylic acid (poly A) and a modified polyuridylic acid strand. This compound is a critical research-grade biochemical tool, primarily valued for its ability to mimic viral double-stranded RNA structures and potently induce interferon production. It is essential for researchers and developers in the fields of immunology, virology, and therapeutic nucleic acid development who require a high-purity, defined-length RNA duplex for studying innate immune responses, antiviral mechanisms, and as a standard in assay development.

Application

• Immunological Research: Used as a potent inducer of interferon (IFN) and other cytokines to study innate immune signaling pathways, particularly through Toll-like receptor 3 (TLR3) and other pattern recognition receptors.
• Virology Studies: Serves as a synthetic analog of viral double-stranded RNA (dsRNA) to investigate viral replication mechanisms, host antiviral defenses, and the development of broad-spectrum antiviral agents.
• Vaccine Adjuvant Research: Explored as a potential immunostimulatory component (adjuvant) in vaccine formulations to enhance immune response efficacy.
• Cell Signaling & Apoptosis: Employed in studies of dsRNA-mediated cellular stress responses, including the activation of protein kinase R (PKR) and pathways leading to apoptosis.
• Diagnostic & Assay Development: Acts as a critical reference standard and positive control in assays designed to detect interferon activity or dsRNA-sensitive cellular responses.
• Therapeutic Nucleic Acid Research: Investigated for its potential in cancer immunotherapy and as a component in novel RNA-based therapeutic strategies.

Basic Information

Product Name	Polyriboadenylic Acid-Polyribo-5-Dimethylaminouridylic Acid
CAS No.	86103-48-2
Molecular Formula	(C10H12N5O7P)n • (C11H15N3O9P)n
Molecular Weight	Variable (Polymer)
Synonyms	Poly(A):Poly(5-Dimethylaminouridylic Acid); Poly A:Poly(5-DMAU); dsRNA Poly A:Poly U Derivative; Polyriboadenylic Acid Complex with Polyribo-5-dimethylaminouridylic Acid; 5-Dimethylaminouridylic Acid Homopolymer Complex with Adenylic Acid Homopolymer; Poly(A)-Poly(5-DMAU); Interferon Inducer dsRNA Analog; Synthetic Double-Stranded RNA Complex
EINECS	Contact for details

Quality Control

Our Polyriboadenylic Acid-Polyribo-5-Dimethylaminouridylic Acid is manufactured under controlled conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including assessment of duplex integrity, strand length distribution, and absence of nucleases. Certificates of Analysis (COA) are provided, detailing specifications for identity, purity (by HPLC), and endotoxin levels, ensuring suitability for sensitive cell-based and biochemical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term stability, store under desiccant.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solid
Identification (IR/UV)	Conforms to reference spectrum
Assay (HPLC)	≥ 90% (as duplex)
Strand Length (n)	Specified range available (e.g., 100-500 bases)
A260/A280 Ratio	1.8 - 2.0
Endotoxin	< 10 EU/mg
Solubility	Soluble in sterile, nuclease-free water or buffer
Biological Activity	Induces interferon production in validated cell lines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.