Description

Hpa I is a high-purity restriction enzyme, a critical tool for precise DNA manipulation in molecular biology research and biotechnological applications. Its specific recognition and cleavage of DNA sequences are fundamental for genetic engineering, cloning, and genomic analysis workflows. This product is essential for pharmaceutical R&D laboratories, academic research institutions, and diagnostic kit manufacturers requiring reliable and consistent enzymatic activity for advanced life science applications.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into plasmid vectors.
• Genotyping and DNA Mapping: Used in restriction fragment length polymorphism (RFLP) analysis for genetic fingerprinting.
• Recombinant DNA Technology: A key reagent for constructing recombinant DNA molecules in biopharmaceutical development.
• Diagnostic Assay Development: Employed in the creation of diagnostic tests that rely on specific DNA sequence detection.
• Genome Editing Support: Used in preparatory steps for techniques like CRISPR to analyze and validate genetic constructs.
• Academic & Industrial Research: A standard tool for fundamental research in genetics, microbiology, and synthetic biology.

Basic Information

Product Name	Hpa I
CAS No.	81295-24-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	HpaI; Endodeoxyribonuclease HpaI; Restriction Endonuclease HpaI; Enzyme Hpa I; Haemophilus parainfluenzae I; Type II Restriction Enzyme HpaI; Deoxyribonuclease HpaI
EINECS	Contact for details

Quality Control

Our Hpa I is manufactured and tested under stringent quality systems to ensure high activity, specificity, and purity, free from contaminating nucleases. Each batch is validated for optimal performance in standard buffer systems. A comprehensive Certificate of Analysis (COA) detailing activity units, purity assessments, and functional testing is provided to guarantee consistency and reliability for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves GTTAAC sequence specifically
Assay (Activity)	≥ 10,000 units/mL
Purity (SDS-PAGE)	≥ 90% single band
Contaminating Nuclease Activity	Not detected
Storage Buffer	10 mM Tris-HCl, pH 7.5, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.