Description

2'-Deoxycytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is a chemically modified nucleotide analog where a non-bridging oxygen atom in the phosphate group is replaced by sulfur, creating a chiral center at phosphorus. This specific Rp-isomer is a critical tool for studying enzyme mechanisms and developing diagnostic assays due to its resistance to hydrolysis by certain phosphatases. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, enzymology, and next-generation sequencing technology development.

Application

• Enzyme Mechanism Studies (Stereochemistry): As a substrate analog to investigate the stereochemical course of phosphoryl transfer reactions catalyzed by polymerases, kinases, and nucleases.
• Single-Molecule Sequencing (SMRT) Technology: Used as a modified nucleotide to monitor DNA synthesis in real-time, enabling long-read, high-accuracy sequencing platforms.
• Diagnostic Assay Development: Employed in the design of probes and assays that require nuclease-resistant nucleotides for enhanced stability and signal detection.
• Structural Biology (X-ray Crystallography): Aids in the structural determination of enzyme active sites by acting as a non-hydrolyzable substrate mimic.
• Antiviral & Anticancer Drug Research: Serves as a precursor or model compound in the development of nucleotide-based therapeutics targeting viral polymerases or cellular DNA replication.
• Biophysical Labeling: The sulfur atom can be used for specific labeling or attachment to surfaces or other molecules for biophysical studies.

Basic Information

Product Name	2'-Deoxycytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt
CAS No.	80951-75-3
Molecular Formula	C9H14N3O13P3S · xNa
Molecular Weight	Approx. 529.2 (Free acid) + (x*22.99) g/mol
Synonyms	dCTPαS, Rp-isomer; 2'-Deoxycytidine-5'-(1-thiotriphosphate), Sp-isomer (Note: IUPAC naming convention can invert Rp/Sp); Deoxycytidine 5'-(α-thiotriphosphate), Rp diastereomer; Rp-dCTPαS; Sodium (Rp)-2'-deoxycytidine-5'-O-(1-thiotriphosphate); Sp-2'-deoxycytidine-5'-O-(1-thiotriphosphate) (alternate naming); α-Thio-dCTP, Rp form; Non-hydrolyzable dCTP analog (Rp)
EINECS	Contact for details

Quality Control

Our 2'-Deoxycytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is produced under stringent conditions to ensure the highest diastereomeric purity and chemical integrity, critical for sensitive biochemical applications. Each batch undergoes comprehensive analysis including HPLC for purity and isomer separation, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided to guarantee reproducible and reliable performance in your research or production processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive). For long-term storage, consider under an inert atmosphere. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Anhydrous basis)
Diastereomeric Purity (Rp-Isomer)	≥ 98%
Water Content (KF)	≤ 5.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin (LAL)	< 10 EU/mg (if required)
Biological Activity Test	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.