Description

5-(And-6)-Carboxyfluorescein [5(6)-FAM] is a high-purity, fluorescent tracer dye widely used as a critical reagent in biochemical and diagnostic applications. Its core value lies in its excellent water solubility and bright green fluorescence upon excitation, making it indispensable for labeling biomolecules. This product is essential for researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors. It serves as a fundamental building block for creating fluorescent probes, conjugates, and assay components.

Application

• Fluorescent Labeling Agent: Primary use for covalently tagging proteins, peptides, antibodies, and oligonucleotides to enable detection and quantification.
• Molecular Biology & Diagnostics: Key component in fluorescence in situ hybridization (FISH) probes, PCR primers, and various assay kits (e.g., ELISA).
• Cell Biology & Microscopy: Used to label cellular components and track biological processes via fluorescence microscopy and flow cytometry.
• Bioconjugation & Probe Synthesis: Serves as the fluorescent moiety in the synthesis of advanced molecular probes and sensor constructs.
• Capillary Electrophoresis & HPLC: Acts as a fluorescent marker or standard in separation and analytical techniques.
• Drug Discovery & High-Throughput Screening (HTS): Utilized in developing fluorescent assays to monitor enzyme activity, receptor binding, and other pharmacological targets.

Basic Information

Product Name	5-(And-6)-Carboxyfluorescein [5(6)-FAM]
CAS No.	80537-70-8
Molecular Formula	C21H12O7
Molecular Weight	376.32 g/mol
Synonyms	5(6)-Carboxyfluorescein; 5(6)-FAM; 5- and 6-Carboxyfluorescein Mixture; FAM Acid; 3',6'-Dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5(6)-carboxylic Acid; Fluorescein-5(6)-carboxylic Acid; 5(6)-Carboxyfluorescein Mixed Isomers
EINECS	Contact for details

Quality Control

Our 5(6)-FAM is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing specific results for purity, absorbance, fluorescence, and other critical parameters is provided with every shipment. We support compliance with relevant industry standards for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥95.0% (Total of 5- and 6- isomers)
Absorbance (λmax)	~492 nm (in 0.1M NaOH)
Fluorescence (λmax)	~518 nm (Excitation ~492 nm)
Water Content (KF)	≤5.0%
Solubility	Freely soluble in dilute aqueous alkali; soluble in DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.