Description

Adenyl-5'-o-Phosphorothioate-(3'-5')Adenosine is a chemically modified dinucleotide featuring a phosphorothioate linkage, which confers enhanced nuclease resistance compared to its natural phosphate counterpart. This property makes it a critical building block and research tool in the development of stable oligonucleotides for therapeutic and diagnostic applications. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, molecular biology, and biotechnology for creating antisense oligonucleotides, aptamers, and other advanced nucleic acid-based products.

Application

• Antisense Oligonucleotide (ASO) Synthesis: As a key phosphorothioate monomer for constructing nuclease-resistant therapeutic oligonucleotides targeting gene expression.
• Aptamer Development: Used in the chemical synthesis of modified aptamers to improve their stability and binding affinity in diagnostic and therapeutic settings.
• Biochemical Research: Serves as a substrate or inhibitor in enzymatic studies to investigate the mechanisms of nucleases, polymerases, and other nucleic acid-interacting proteins.
• Probe and Primer Modification: Incorporation into DNA/RNA probes and primers to enhance their stability against degradation in demanding assay conditions.
• Pharmaceutical Intermediate: A vital raw material in the GMP-compliant production of oligonucleotide-based active pharmaceutical ingredients (APIs).
• Structural Biology Studies: Used to prepare modified nucleic acid crystals for X-ray crystallography to study backbone interactions and conformations.

Basic Information

Product Name	Adenyl-5'-o-Phosphorothioate-(3'-5')Adenosine
CAS No.	80186-83-0
Molecular Formula	C20H25N10O11P2S
Molecular Weight	675.5 g/mol
Synonyms	Ap(s)A; P1,P2-Di(adenosine-5') thiopyrophosphate; Adenosine-5'-thiopyrophosphoryl-5'-adenosine; 5',5'''-Thiopyrophosphoryl-diadenosine; Diadenosine 5',5'''-P1,P2-thiopyrophosphate; 5'-Adenylyl thiophosphoryl-5'-adenosine; Adenosine 5'-(trihydrogen thiopyrophosphate) 5'-5''-ester with adenosine; dApsA (commonly used abbreviation)
EINECS	Contact for details

Quality Control

Our Adenyl-5'-o-Phosphorothioate-(3'-5')Adenosine is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the exacting standards required for pharmaceutical and life science research. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment to guarantee traceability, consistency, and compliance with your research or production protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, it is recommended to seal under argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.