Description

Methidiumpropyl Edta CAS NO 80082-09-3 is a specialized chelating agent and nucleic acid stain, known for its high-affinity binding to DNA. This compound is critical for applications requiring the inhibition of nucleases and the visualization of nucleic acids in various analytical techniques. It is an essential reagent for molecular biology research, diagnostic assay development, and biochemical analysis in pharmaceutical and biotechnology sectors.

Application

• Molecular Biology Research: Used as a potent nuclease inhibitor in DNA extraction and purification protocols to prevent degradation.
• Gel Electrophoresis: Employed as a fluorescent stain for visualizing DNA bands in agarose and polyacrylamide gels.
• Diagnostic Assays: Serves as a key component in diagnostic kits for detecting specific DNA sequences.
• Biochemical Studies: Utilized in studies of DNA-protein interactions and chromatin structure due to its intercalating properties.
• Pharmaceutical Development: Applied in quality control and research for nucleic acid-based therapeutics.
• Forensic Analysis: Aids in DNA staining for sample analysis and identification.

Basic Information

Product Name	Methidiumpropyl Edta
CAS No.	80082-09-3
Molecular Formula	C28H42N6O8
Molecular Weight	590.67 g/mol
Synonyms	MPE; Methidiumpropyl-EDTA; MPE-EDTA; Ethylenediaminetetraacetic acid methidiumpropyl ester; 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium ethylenediaminetetraacetate; Phenanthridinium, 3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenyl-, salt with N,N'-1,2-ethanediylbis[N-(carboxymethyl)glycine] (1:1)
EINECS	Contact for details

Quality Control

Our Methidiumpropyl Edta is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets high-purity research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	Dark red to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.