Description

Pentafluorobenzenesulfonyl Fluorescein is a specialized fluorescent labeling reagent and chemical intermediate. This compound is valued for its unique combination of a highly reactive pentafluorobenzenesulfonyl (PBSF) group and the fluorescein fluorophore, enabling sensitive detection and conjugation. It is primarily needed by researchers and manufacturers in the fields of analytical chemistry, biochemistry, and pharmaceutical development for creating advanced probes and diagnostic agents.

Application

• Fluorescent Labeling Reagent: Used for the derivatization and sensitive detection of amines, alcohols, and thiols in HPLC and capillary electrophoresis.
• Bioconjugation Chemistry: Serves as a key intermediate for synthesizing fluorescently tagged antibodies, proteins, and peptides for bioimaging and flow cytometry.
• Proteomics Research: Employed in activity-based protein profiling (ABPP) and the development of fluorescent sensors for enzyme activity.
• Diagnostic Assay Development: A critical component in the manufacture of fluorescent probes and kits for clinical diagnostics and high-throughput screening.
• Material Science: Used in the surface functionalization of nanoparticles and polymers to introduce fluorescent properties for tracking and analysis.
• Chemical Biology Tool: Facilitates the study of biomolecular interactions and cellular processes through targeted fluorescent labeling.

Basic Information

Item	Details
Product Name	Pentafluorobenzenesulfonyl Fluorescein
CAS No.	728912-45-6
Molecular Formula	C25H11F5O7S
Molecular Weight	574.41 g/mol
Synonyms	PBSF-Fluorescein; Fluorescein Pentafluorobenzenesulfonate; 5(6)-Carboxyfluorescein Pentafluorobenzenesulfonyl Ester; Pentafluorophenylsulfonyl Fluorescein; 2-(3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(or 6)-yl)carbonyl-pentafluorobenzenesulfonate; 5(6)-FAM-PBSF Ester; Fluorescein-based PBSF Labeling Reagent
EINECS	Contact for details

Quality Control

Our Pentafluorobenzenesulfonyl Fluorescein is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and Mass Spectrometry, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with complete chromatographic data are provided to guarantee reproducible performance and lot-to-lot consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.