Description

5-Ethynyl-2'-Deoxyuridylic Acid is a modified nucleoside monophosphate derivative, a crucial building block in nucleic acid chemistry and molecular biology research. Its unique ethynyl group provides a versatile handle for bioorthogonal "click chemistry" reactions, enabling precise labeling and conjugation of oligonucleotides. This compound is essential for researchers and manufacturers in the fields of diagnostic probe development, antisense oligonucleotide synthesis, and next-generation sequencing technologies.

Application

• Diagnostic Probe Synthesis: Used as a modified nucleotide for creating highly specific fluorescent or affinity-labeled DNA/RNA probes for in situ hybridization (FISH) and PCR-based diagnostics.
• Antisense & Therapeutic Oligonucleotide Development: Incorporated into oligonucleotide backbones to enable site-specific conjugation with targeting ligands, reporter molecules, or drug payloads.
• Click Chemistry Labeling: The terminal alkyne (ethynyl) group serves as a reactive site for copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-functionalized dyes, biotin, or other molecules.
• Next-Generation Sequencing (NGS): Acts as a substrate for the synthesis of specialized nucleotides used in sequencing-by-synthesis platforms and single-molecule sequencing.
• Bioconjugation & Biomaterial Fabrication: Facilitates the covalent attachment of oligonucleotides to surfaces, nanoparticles, polymers, or proteins for biosensor and microarray development.
• Structural Biology Studies: Employed to introduce stable, minimally disruptive modifications into nucleic acids for NMR, X-ray crystallography, and other biophysical analyses.

Basic Information

Item	Detail
Product Name	5-Ethynyl-2'-Deoxyuridylic Acid
CAS No.	79762-80-4
Molecular Formula	C12H13N2O8P
Molecular Weight	344.21 g/mol
Synonyms	5-Ethynyl-2'-deoxyuridine 5'-monophosphate; 5-EdUMP; 5-Ethynyl-dUMP; 2'-Deoxy-5-ethynyluridylic acid; 5-Ethynyl-2'-deoxyuridine monophosphate; 5-Ethynyl-dUridine 5'-phosphate; 5-Ethynyl-2'-deoxyuridine 5'-(dihydrogen phosphate)
EINECS	Contact for details

Quality Control

Our 5-Ethynyl-2'-Deoxyuridylic Acid is produced under stringent quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the exacting standards required for life science research and development. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.