Description

D-Ile-Phe-Lys p-Nitroanilide CAS NO 76626-41-0 is a specialized tripeptide chromogenic substrate designed for high-sensitivity enzymatic assays. Its core value lies in providing a reliable and quantifiable signal for the detection and kinetic study of specific proteolytic enzymes. This compound is essential for researchers and quality control professionals in the pharmaceutical and life sciences industries who require precise biochemical tools. It serves as a critical reagent for investigating enzyme mechanisms, screening inhibitors, and developing diagnostic assays.

Application

• Protease and Enzyme Activity Assays: Primary substrate for the spectrophotometric or fluorometric determination of trypsin, plasmin, and related serine proteases.
• Biochemical Research: Used in kinetic studies to determine enzyme parameters such as Km and Vmax, and to study enzyme inhibition mechanisms.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic protease inhibitors.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical analysis of blood coagulation and fibrinolysis pathways.
• Quality Control in Bioprocessing: Monitors the activity and consistency of enzyme batches used in industrial biotechnological processes.

Basic Information

Product Name	D-Ile-Phe-Lys p-Nitroanilide
CAS No.	76626-41-0
Molecular Formula	C30H42N6O6
Molecular Weight	582.70 g/mol
Synonyms	D-Isoleucyl-L-phenylalanyl-L-lysine 4-nitroanilide; Ile-Phe-Lys-pNA; H-D-Ile-L-Phe-L-Lys-pNA; D-Ile-Phe-Lys-pNA; Chromogenic Substrate for Trypsin; Boc-D-Ile-L-Phe-L-Lys-pNA; Protease Substrate 76626-41-0; N-(Benzyloxycarbonyl)-D-isoleucyl-L-phenylalanyl-N6-(4-nitrobenzoyl)-L-lysine amide
EINECS	Contact for details

Quality Control

Every batch of D-Ile-Phe-Lys p-Nitroanilide is manufactured under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure they meet the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with each shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.